Stabilization and activity-enhancement of mandelate racemase from Pseudomonas putida ATCC 12336 by immobilization

被引：16

作者：

Strauss, UT ^{[1
]}

Kandelbauer, A ^{[1
]}

Faber, K ^{[1
]}

机构：

[1] Graz Univ, Inst Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 06期

关键词：

activation; immobilization; mandelate racemase [EC 5.1.2.2; stabilization;

D O I：

10.1023/A:1005621021983

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mandelate racemase [EC 5.1.2.2] from Pseudomonas putida ATCC 12336 was efficiently immobilized through ionic binding onto DEAE- and TEAE 23-cellulose. The activity of the immobilized enzyme was significantly enhanced as compared to the native protein, i.e., 2.7- and 2.5-fold, respectively. DEAE-cellulose-immobilized mandelate racemase could be efficiently used in repeated batch reactions for the racemization of (R)-mandelic acid under mild conditions.

引用

页码：515 / 520

页数：6

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