Stabilization and activity-enhancement of mandelate racemase from Pseudomonas putida ATCC 12336 by immobilization

被引：16
|
作者：
Strauss, UT [1 ]
Kandelbauer, A [1 ]
Faber, K [1 ]
机构：
[1] Graz Univ, Inst Chem Organ & Bioorgan Chem, A-8010 Graz, Austria
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 06期
关键词：
activation; immobilization; mandelate racemase [EC 5.1.2.2; stabilization;
D O I：
10.1023/A:1005621021983
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Mandelate racemase [EC 5.1.2.2] from Pseudomonas putida ATCC 12336 was efficiently immobilized through ionic binding onto DEAE- and TEAE 23-cellulose. The activity of the immobilized enzyme was significantly enhanced as compared to the native protein, i.e., 2.7- and 2.5-fold, respectively. DEAE-cellulose-immobilized mandelate racemase could be efficiently used in repeated batch reactions for the racemization of (R)-mandelic acid under mild conditions.
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页码：515 / 520
页数：6
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