In vivo circular RNA production using a constitutive promoter for high-level expression

被引：9
|
作者：
Umekage, So [1 ]
Kikuchi, Yo [1 ]
机构：
[1] Toyohashi Univ Technol, Div Life Sci & Biotechnol, Dept Ecol Engn, Aichi 4418580, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 108卷 / 04期
关键词：
Circularization; Permuted intron-exon; Aptamer; In vivo expression; Constitutive promoter; ACTIVATED HIV-1 TRANSCRIPTION; ESCHERICHIA-COLI; INTRON; SEQUENCES; COMPLEX; GENE;
D O I：
10.1016/j.jbiosc.2009.04.011
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：354 / 356
页数：3
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