Characterisation of Antibodies for the Immunochemical Detection of Enterobacter sakazakii

被引：19
|
作者：
Hochel, Igor [1 ]
Skvor, Jiri [2 ]
机构：
[1] Prague Inst Chem Technol, Fac Food & Biochem Technol, Dept Biochem & Microbiol, CR-16628 Prague, Czech Republic
[2] Charles Univ Prague, Dept Med Biophys, Fac Med 1, Prague, Czech Republic
来源：
CZECH JOURNAL OF FOOD SCIENCES | 2009年 / 27卷
关键词：
Enterobacter sakazakii; heat-labile antigen; IgG; ELISA; cross-reactivity; DUBLINENSIS SP-NOV; INFECTION; PROPOSAL; ELISA; GENUS;
D O I：
10.17221/206/2009-CJFS
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739(T) were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 x 10(5) cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.
引用
收藏
页码：66 / 74
页数：9
相关论文
共 50 条
  • [11] Vaginal infection by Enterobacter sakazakii
    Ongrádi, J
    SEXUALLY TRANSMITTED INFECTIONS, 2002, 78 (06) : 467 - 467
  • [12] Nosocomial Enterobacter sakazakii infections
    Lai, KK
    Naravane, SR
    INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 1998, 19 (09): : 709 - 709
  • [13] Enterobacter sakazakii infection in the newborn
    Bar-Oz, B
    Preminger, A
    Peleg, O
    Block, C
    Arad, I
    ACTA PAEDIATRICA, 2001, 90 (03) : 356 - 358
  • [14] Thermobacteriological characterization of Enterobacter sakazakii
    Arroyo, C.
    Condon, S.
    Pagan, R.
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 136 (01) : 110 - 118
  • [15] A medium for the presumptive detection of Enterobacter sakazakii in infant formula:: Interlaboratory study
    Leuschner, RGK
    Bew, J
    Alfonsina, F
    Blanco, C
    Bräunig, J
    Brookes, F
    Friedl, E
    Gooding, A
    Hahn, G
    Hudson, C
    Reid, TMS
    Short, A
    Souza, M
    Stegeman, H
    Talbot, H
    Veleta, K
    Withers, Z
    Wood, M
    JOURNAL OF AOAC INTERNATIONAL, 2004, 87 (03) : 604 - 613
  • [16] Ultrasensitive Detection of Viable Enterobacter sakazakii by a Continual Cascade Nanozyme Biosensor
    Zhang, Li
    Chen, Yuting
    Cheng, Nan
    Xu, Yuancong
    Huang, Kunlun
    Luo, Yunbo
    Wang, Peixia
    Duan, Demin
    Xu, Wentao
    ANALYTICAL CHEMISTRY, 2017, 89 (19) : 10194 - 10200
  • [17] A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples
    Guillaume-Gentil, O
    Sonnard, V
    Kandhai, MC
    Marugg, JD
    Joosten, H
    JOURNAL OF FOOD PROTECTION, 2005, 68 (01) : 64 - 69
  • [18] A medium for the presumptive detection of Enterobacter sakazakii in infant formula: Interlaboratory study
    Leuschner, R.G.K. (renata.leuschner@cec.eu.int), 1600, AOAC International (87):
  • [19] Enterobacter sakazakii -: new foods for thought?
    Farber, JM
    LANCET, 2004, 363 (9402): : 5 - 6
  • [20] ENTEROBACTER-SAKAZAKII MENINGITIS IN NEONATES
    WILLIS, J
    ROBINSON, JE
    PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1988, 7 (03) : 196 - 199
12345