Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera: Culicidae)

被引:0
|
作者
Rutledge, CR [1 ]
Wesson, DM
Meek, CL
机构
[1] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70893 USA
[2] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Trop Med, New Orleans, LA 70126 USA
关键词
Anopheles quadrimaculatus; An; inundatus; immature stages; polymerase chain reaction;
D O I
暂无
中图分类号
Q96 [昆虫学];
学科分类号
摘要
A polymerase chain reaction assay that detects differences in the 2nd internal transcribed spacer of ribosomal DNA was tested as a means to identify all immature stages of 2 species in the Anopheles quadrimaculatus sibling species complex. The technique was successful in amplifying DNA from eggs, 1st-4th instars, and pupae of An. quadrimaculatus Say and An. inundatus Reinert.
引用
收藏
页码:573 / 575
页数:3
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