SIMULATION OF ASTROCYTIC CALCIUM DYNAMICS IN LATTICE LIGHT SHEET MICROSCOPY IMAGES

被引：2

作者：

Badoual, Anais ^{[1
]}

Arizono, Misa ^{[2
]}

Denizot, Audrey ^{[3
]}

Ducros, Mathieu ^{[4
]}

Berry, Hugues ^{[5
,6
]}

Nagerl, U. Valentin ^{[2
]}

Kervrann, Charles ^{[1
]}

机构：

[1] Inria, Serp Team, Ctr Rech Bretagne Atlantique, Rennes, France

[2] Interdisciplinary Inst Neurosci, UMR5297, CNRS, Bordeaux, France

[3] Okinawa Inst Sci & Technol, Computat Neurosci Unit, Onna, Japan

[4] Bordeaux Imaging Ctr, UMS3420 CNRS, US04 INSERM, Bordeaux, France

[5] Inria, Ctr Rech Rhone Alpes, Beagle Team, F-69603 Villeurbanne, France

[6] Univ Lyon, LIRIS, UMR5205, CNRS, F-69621 Villeurbanne, France

来源：

2021 IEEE 18TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2021年

关键词：

Astrocytes; calcium signals; image analysis; lattice light sheet microscopy; synthetic training datasets;

D O I：

10.1109/ISBI48211.2021.9433984

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Astrocytes regulate neuronal information processing through a variety of spatio-temporal calcium signals. Recent advances in calcium imaging have started to shine light on astrocytic activity, but the complexity and size of the recorded data strongly call for more advanced computational analysis tools. Their development is currently hindered by the lack of reliable, labeled annotations that are essential for the evaluation of algorithms and the training of learning-based methods. To solve this labeling problem, we have designed a generator of 2D/3D lattice light sheet microscopy (LLSM) sequences which realistically depict the calcium dynamics of astrocytes. By closely modeling calcium kinetics in real astrocytic ramifications, the generated datasets open the door for the deployment of convolutional neural networks in LLSM.

引用

页码：135 / 139

页数：5

共 50 条

[21] Lattice Light-Sheet Microscopy with Incoherent Detection and Extended Field of View
Potcoava, Mariana C.
Mann, Christopher J.
Alford, Simon T.
Art, Jonathan
ADVANCES IN MICROSCOPIC IMAGING III, 2021, 11922
[22] Enhanced axial resolution of lattice light sheet microscopy by fluorescence differential detection
Gan, Yanhong
Ma, Ye
Gong, Wenwen
Liu, Wenjie
Wang, Ziang
Hao, Xiang
Han, Yubing
Kuang, Cuifnag
Liu, Xu
OPTICS EXPRESS, 2022, 30 (15) : 27381 - 27394
[23] Imaging mitotic processes in three dimensions with lattice light-sheet microscopy
Mimori-Kiyosue, Yuko
CHROMOSOME RESEARCH, 2021, 29 (01) : 37 - 50
[24] Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy
Sena, Giovanni
Frentz, Zak
Birnbaum, Kenneth D.
Leibler, Stanislas
PLOS ONE, 2011, 6 (06):
[25] Immunoimaging with Light Sheet Microscopy: Microglial Dynamics in the Developing Zebrafish Brain
Holland, Daniel B.
Truong, Thai V.
Junge, Jason A.
Fraser, Scott E.
BIOPHYSICAL JOURNAL, 2016, 110 (03) : 148A - 148A
[26] Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
Rosenberg, Jillian
Huang, Jun
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (155):
[27] Hyperspectral light sheet microscopy
Jahr, Wiebke
Schmid, Benjamin
Schmied, Christopher
Fahrbach, Florian O.
Huisken, Jan
NATURE COMMUNICATIONS, 2015, 6
[28] Hyperspectral light sheet microscopy
Wiebke Jahr
Benjamin Schmid
Christopher Schmied
Florian O. Fahrbach
Jan Huisken
Nature Communications, 6
[29] Light sheet fluorescence microscopy
Willson, Joseph
NATURE REVIEWS METHODS PRIMERS, 2021, 1 (01):
[30] Light sheet fluorescence microscopy
Nature Reviews Methods Primers, 1

← 1 2 3 4 5 →