Solid-phase enrichment and analysis of electrophilic natural products

被引：2
|
作者：
Wesche, Frank [1 ]
He, Yue [1 ]
Bode, Helge B. [1 ,2 ]
机构：
[1] Goethe Univ Frankfurt, Fachbereich Biowissensch, Merck Stiftungsprof Mol Biotechnol, Max von Laue Str 9, D-60438 Frankfurt, Germany
[2] Goethe Univ Frankfurt, Buchmann Inst Mol Life Sci BMLS, Max von Laue Str 15, D-60438 Frankfurt, Germany
来源：
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY | 2017年 / 13卷
基金：
欧洲研究理事会;
关键词：
azides; click chemistry; enrichment; electrophilic natural products; epoxides; glidobactin; Photorhabdus; stilbenes; DISCOVERY; PROBES;
D O I：
10.3762/bjoc.13.43
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
In search for new natural products, which may lead to the development of new drugs for all kind of applications, novel methods are needed. Here we describe the identification of electrophilic natural products in crude extracts via their reactivity against azide as a nucleophile followed by their subsequent enrichment using a cleavable azide-reactive resin (CARR). Using this approach, natural products carrying epoxides and alpha,beta-unsaturated enones as well as several unknown compounds were identified in crude extracts from entomopathogenic Photorhabdus bacteria.
引用
收藏
页码：405 / 409
页数：5
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