High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

被引：11
|
作者：
Ruiz-Tortola, Angela [1 ]
Prats-Quilez, Francisco [1 ]
Gonzalez-Lucas, Daniel [2 ]
Banuls, Maria-Jose [2 ]
Maquieira, Angel [2 ]
Wheeler, Guy [3 ]
Dalmay, Tamas [3 ]
Griol, Amadeu [1 ]
Hurtado, Juan [1 ]
Garcia-Ruperez, Jaime [1 ]
机构：
[1] Univ Politecn Valencia, Nanophoton Technol Ctr, Camino Vera S-N, E-46022 Valencia, Spain
[2] Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Dept Quim, IDM, E-46022 Valencia, Spain
[3] Univ East Anglia, Sch Biol Sci, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England
来源：
BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 04期
基金：
欧盟地平线“2020”;
关键词：
MACH-ZEHNDER INTERFEROMETER; MICRORING RESONATORS; MULTIPLEXED DETECTION; OPTICAL BIOSENSORS; BINDING-KINETICS; SLOW-LIGHT; RNA; CANCER; HYBRIDIZATION; EXPRESSION;
D O I：
10.1364/BOE.9.001717
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：1717 / 1727
页数：11
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