A Novel 6′-N-Aminoglycoside Acetyltransferase, AAC(6′)-Ial, from a Clinical Isolate of Serratia marcescens

被引：5
|
作者：
Tada, Tatsuya [1 ]
Miyoshi-Akiyama, Tohru [2 ]
Shimada, Kayo [1 ]
Dahal, Rajan K. [3 ]
Mishra, Shyam K. [3 ]
Ohara, Hiroshi [4 ]
Kirikae, Teruo [1 ]
Pokhrel, Bharat M. [3 ]
机构：
[1] Natl Ctr Global Hlth & Med, Res Inst, Dept Infect Dis, Tokyo 1628655, Japan
[2] Natl Ctr Global Hlth & Med, Res Inst, Pathogen Microbe Lab, Tokyo 1628655, Japan
[3] Tribhuvan Univ, Inst Med, Dept Microbiol, Kathmandu, Nepal
[4] Natl Ctr Global Hlth & Med, Dept Int Med Cooperat, Tokyo 1628655, Japan
来源：
MICROBIAL DRUG RESISTANCE | 2016年 / 22卷 / 02期
关键词：
RIBOSOMAL-RNA METHYLASE; METALLO-BETA-LACTAMASE; PSEUDOMONAS-AERUGINOSA; IDENTIFICATION; RESISTANCE; AMINOGLYCOSIDES; OUTBREAK; GENES; KOREA;
D O I：
10.1089/mdr.2015.0126
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Serratia marcescens IOMTU115 has a novel 6 '-N-aminoglycoside acetyltransferase-encoding gene, aac(6 ')-Ial. The encoded protein AAC(6 ')-Ial has 146 amino acids, with 91.8% identity to the amino acid sequence of AAC(6 ')-Ic in S. marcescens SM16 and 97.3% identity to the amino acid sequence of AAC(6 ')-Iap in S. marcescens WW4. The minimum inhibitory concentrations of aminoglycosides for Escherichia coli expressing AAC(6 ')-Ial were similar to those for E. coli expressing AAC(6 ')-Ic or AAC(6 ')-Iap. Thin-layer chromatography showed that AAC(6 ')-Ial, AAC(6 ')-Ic, or AAC(6 ')-Iap acetylated all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin. Kinetics assays revealed that AAC(6 ')-Ial is a functional acetyltransferase against aminoglycosides. The aac(6 ')-Ial gene was located on chromosomal DNA.
引用
收藏
页码：103 / 108
页数：6
相关论文
共 50 条
  • [41] Novel 3-N-aminoglycoside acetyltransferase gene, aac(3)-Ic, from a Pseudomonas aeruginosa integron
    Riccio, ML
    Docquier, JD
    Dell'Amico, E
    Luzzaro, F
    Amicosante, G
    Rossolini, GM
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (05) : 1746 - 1748
  • [42] Overexpression and characterization of the chromosomal aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium
    Wright, GD
    Ladak, P
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (05) : 956 - 960
  • [43] Identification of a small molecule inhibitor of the aminoglycoside 6′-N-acetyltransferase type Ib [AAC(6′)-Ib] using mixture-based combinatorial libraries
    Tung Tran
    Chiem, Kevin
    Jani, Saumya
    Arivett, Brock A.
    Lin, David L.
    Lad, Rupali
    Jimenez, Veronica
    Farone, Mary B.
    Debevec, Ginamarie
    Santos, Radleigh
    Giulianotti, Marc
    Pinilla, Clemencia
    Tolmasky, Marcelo E.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2018, 51 (05) : 752 - 761
  • [44] Spread of novel aminoglycoside resistance gene aac(6′)-Iad among Acinetobacter clinical isolates in Japan
    Doi, Y
    Wachino, J
    Yamane, K
    Shibata, N
    Yagi, T
    Shibayama, K
    Kato, H
    Arakawa, Y
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (06) : 2075 - 2080
  • [45] Inhibition of Aminoglycoside 6′-N-acetyltransferase Type Ib (AAC(6′)-Ib): Structure-Activity Relationship of Substituted Pyrrolidine Pentamine Derivatives as Inhibitors
    Rocha, Kenneth
    Magallon, Jesus
    Reeves, Craig
    Phan, Kimberly
    Vu, Peter
    Oakley-Havens, Crista L.
    Kwan, Stella
    Ramirez, Maria Soledad
    LaVoi, Travis
    Donow, Haley
    Chapagain, Prem
    Santos, Radleigh
    Pinilla, Clemencia
    Giulianotti, Marc A.
    Tolmasky, Marcelo E.
    BIOMEDICINES, 2021, 9 (09)
  • [46] Acetylation of aminoglycoside antibiotics with 6′-methylamino group, istamycin B and micronomicin, by a novel aminoglycoside 6′-acetyltransferase of actinomycete origin
    Sunada, A
    Ikeda, Y
    Kondo, S
    Hotta, K
    JOURNAL OF ANTIBIOTICS, 2000, 53 (12): : 1416 - 1419
  • [47] Design and synthesis of inhibitors of aminoglycoside N-6′-acetyltransferase.
    Auclair, K
    Caputo, C
    Kolin, D
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 224 : U42 - U42
  • [48] Understanding and overcoming aminoglycoside resistance caused by N-6′-acetyltransferase
    Vong, Kenward
    Auclair, Karine
    MEDCHEMCOMM, 2012, 3 (04) : 397 - 407
  • [49] AAC(3)-XI, a New Aminoglycoside 3-N-Acetyltransferase from Corynebacterium striatum
    Galimand, Marc
    Fishovitz, Jennifer
    Lambert, Thierry
    Barbe, Valerie
    Zajicek, Jaroslav
    Mobashery, Shahriar
    Courvalin, Patrice
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2015, 59 (09) : 5647 - 5653
  • [50] Identification of a Serratia marcescens Clinical Isolate with Multiple Quinolone Resistance Mechanisms from China
    Yang, Hai-Fei
    Cheng, Jun
    Hu, Li-Fen
    Ye, Ying
    Li, Jia-Bin
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (10) : 5426 - 5427
12345