Molecularly imprinted cryogels for carbonic anhydrase purification from bovine erythrocyte

被引：12
|
作者：
Uygun, Murat [1 ]
Karagozler, A. Alev [2 ]
Denizli, Adil [3 ]
机构：
[1] Adnan Menderes Univ, Kocarli Vocat & Training Sch, Aydin, Turkey
[2] Adnan Menderes Univ, Fac Arts & Sci, Dept Chem, Aydin, Turkey
[3] Hacettepe Univ, Fac Sci, Dept Chem, Ankara, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2014年 / 42卷 / 02期
关键词：
carbonic anhydrase; cryogel; molecularly imprinted polymer; purification; POLY(HYDROXYETHYL METHACRYLATE) CRYOGEL; IMMUNOGLOBULIN-G; ALBUMIN DEPLETION; CHROMATOGRAPHY; REMOVAL; SEPARATION;
D O I：
10.3109/21691401.2013.864663
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.16 mg/g. Selectivity of the molecularly imprinted cryogel was investigated using albumin, hemoglobin, IgG, gamma-globulin, and lysozyme as competitor proteins and selectivity ratios were found to be 15.26, 60.05, 21.88, 17.61, and 17.42, respectively. Carbonic anhydrase purity was demonstrated by SDS-PAGE and zymogram results.
引用
收藏
页码：128 / 137
页数：10
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