CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms

被引：68
|
作者：
Shapiro, Rebecca S. [1 ,2 ,3 ]
Chavez, Alejandro [4 ]
Collins, James J. [1 ,2 ,5 ]
机构：
[1] MIT, Dept Biol Engn, Inst Med Engn & Sci, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA
[2] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
[3] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON, Canada
[4] Columbia Univ Coll Phys & Surg, Dept Pathol & Cell Biol, 630 W 168th St, New York, NY 10032 USA
[5] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA
来源：
NATURE REVIEWS MICROBIOLOGY | 2018年 / 16卷 / 06期
关键词：
HOMOLOGOUS RECOMBINATION; FUNCTIONAL GENOMICS; RNA INTERFERENCE; GENE REPLACEMENT; PLASMODIUM; DNA; TOXOPLASMA; YEAST; ACHIEVEMENTS; DETERMINANTS;
D O I：
10.1038/s41579-018-0002-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Genetic manipulation of microorganisms has been crucial in understanding their biology, yet for many microbial species, robust tools for comprehensive genetic analysis were lacking until the advent of CRISPR-Cas-based gene editing techniques. In this Progress article, we discuss advances in CRISPR-based techniques for the genetic analysis of genetically intractable microorganisms, with an emphasis on mycobacteria, fungi and parasites. We discuss how CRISPR-based analyses in these organisms have enabled the discovery of novel gene functions, the investigation of genetic interaction networks and the identification of virulence factors.
引用
收藏
页码：333 / 339
页数：7
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