Essential role of Pro 74 in stefin B amyloid-fibril formation: Dual action of cyclophilin A on the process

被引：18

作者：

Smajlovic, Aida ^{[4
]}

Berbic, Selma ^{[4
]}

Schiene-Fischer, Cordelia ^{[3
]}

Tusek-Znidaric, Magda ^{[2
]}

Taler, Ajda ^{[1
]}

Jenko-Kokalj, Sasa ^{[1
]}

Turk, Dusan ^{[1
]}

Zerovnik, Eva ^{[1
]}

机构：

[1] Jozef Stefan Inst, Dept Biochem & Mol Struct Biol, Ljubljana 1000, Slovenia

[2] Natl Inst Biol, Dept Plant Physiol & Biotechnol, Ljubljana 1000, Slovenia

[3] Max Planck Res Unit Enzymol Prot Folding, D-06120 Halle, Saale, Germany

[4] Univ Tuzla, Farmaceut Fac, Dept Biochem, Tuzla 75000, Bosnia & Herceg

来源：

FEBS LETTERS | 2009年 / 583卷 / 07期

关键词：

Stefin B; Amyloid fibril; Proline cis/trans isomerism; Cyclophilin A; Kinetics of fibrillation; Protein-protein interaction; NUCLEAR-MAGNETIC-RESONANCE; ESCHERICHIA-COLI; IN-VITRO; PROTEIN; DOMAIN; CYSTATIN; DISEASE; INTERMEDIATE; AGGREGATION; OLIGOMERS;

D O I：

10.1016/j.febslet.2009.02.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that Pro74 in human stefin B is critical for fibril formation and that proline isomerization plays an important role. The stefin B P74S mutant did not fibrillate over the time of observation at 25 degrees C, and it exhibited a prolonged lag phase at 30 degrees C and 37 degrees C. The peptidyl prolyl cis/trans isomerase cyclophilin A, when added to the wild-type protein, exerted two effects: it prolonged the lag phase and increased the yield and length of the fibrils. Addition of the inactive cyclophilin A R55A variant still resulted in a prolonged lag phase but did not mediate the increase of the final fibril yield. These results demonstrate that peptidyl prolyl cis/trans isomerism is rate-limiting in stefin B fibril formation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1114 / 1120

页数：7

共 13 条

[11] Essential role of proline isomerization in stefin B tetramer formation
Kokalj, Sasa Jenko
Guncar, Gregor
Stern, Igor
Morgan, Gareth
Rabzelj, Sabina
Kenig, Manca
Staniforth, Rosemary A.
Waltho, Jonathan P.
Zerovnik, Eva
Turk, Dusan
JOURNAL OF MOLECULAR BIOLOGY, 2007, 366 (05) : 1569 - 1579
[12] Amyloid fibril formation by human stefin B: influence of the initial pH-induced intermediate state
Zerovnik, E
Turk, V
Waltho, JP
BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 : 543 - 547
[13] Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B (vol 10, 1471, 2021)
Jahic Mujkic, Alma
Tusek Znidaric, Magda
Berbic, Selma
Zerovnik, Eva
ANTIOXIDANTS, 2024, 13 (01)

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