Glycol methacrylate embedding for light microscopy: Basic principles and trouble-shooting

被引：31
|
作者：
Gerrits, PO [1 ]
Horobin, RW [1 ]
机构：
[1] UNIV SHEFFIELD, DEPT BIOMED SCI, SHEFFIELD S10 2TN, S YORKSHIRE, ENGLAND
来源：
JOURNAL OF HISTOTECHNOLOGY | 1996年 / 19卷 / 04期
关键词：
acrylic resins; embedding; glycol methacrylate; immunohistochemistry; tissue processing;
D O I：
10.1179/his.1996.19.4.297
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Acrylic resin mixtures are now widely used as embedding media for the preparation of tissue sections. Most of these mixtures are based on 2-hydroxyethyl methacrylate (glycol methacrylate, GMA). Resin embedding preserves tissue components far better than paraffin, celloidin or frozen sections. The present review describes the basic principles and trouble shooting, in particular: the chemical and physical properties of GMA, and components used for GMA mixtures; fixation of tissues for resin embedding; methods for dehydration; microtomy; stretching on water and mounting in relation to the final dimensions of GMA sections; staining of GMA 3embedded tissue sections; and the use of GMA resins in immunohistochemistry. In addition, standard, step by step procedures for embedding tissues in GMA is included.
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页码：297 / 311
页数：15
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