Glycol methacrylate embedding for light microscopy: Basic principles and trouble-shooting

被引：31

作者：

Gerrits, PO ^{[1
]}

Horobin, RW ^{[1
]}

机构：

[1] UNIV SHEFFIELD, DEPT BIOMED SCI, SHEFFIELD S10 2TN, S YORKSHIRE, ENGLAND

来源：

JOURNAL OF HISTOTECHNOLOGY | 1996年 / 19卷 / 04期

关键词：

acrylic resins; embedding; glycol methacrylate; immunohistochemistry; tissue processing;

D O I：

10.1179/his.1996.19.4.297

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Acrylic resin mixtures are now widely used as embedding media for the preparation of tissue sections. Most of these mixtures are based on 2-hydroxyethyl methacrylate (glycol methacrylate, GMA). Resin embedding preserves tissue components far better than paraffin, celloidin or frozen sections. The present review describes the basic principles and trouble shooting, in particular: the chemical and physical properties of GMA, and components used for GMA mixtures; fixation of tissues for resin embedding; methods for dehydration; microtomy; stretching on water and mounting in relation to the final dimensions of GMA sections; staining of GMA 3embedded tissue sections; and the use of GMA resins in immunohistochemistry. In addition, standard, step by step procedures for embedding tissues in GMA is included.

引用

页码：297 / 311

页数：15

共 25 条

[1] BASIC PRINCIPLES ESSENTIAL TO EFFECTIVE TROUBLE-SHOOTING
LEUGNER, L
CANADIAN MINING JOURNAL, 1985, 106 (09) : S5 - S5
[2] Application of Basic Principles of Physics to Head and Neck MR Angiography: Trouble-shooting for Artifacts
Pandey, Shilpa
Hakky, Michael
Kwak, Ellie
Jara, Hernan
Geyer, Carl A.
Erbay, Sami H.
RADIOGRAPHICS, 2013, 33 (03) : E113 - E123
[3] GLYCOL METHACRYLATE EMBEDDING AND MICROWAVE STAINING FOR LIGHT-MICROSCOPY OF THE MOUSE COCHLEA
KATBAMNA, B
RALSTON, A
SCANNING MICROSCOPY, 1994, 8 (02) : 345 - 350
[4] USE OF METHACRYLATE EMBEDDING IN LIGHT MICROSCOPY
FLAX, MH
CAULFIELD, JB
ARCHIVES OF PATHOLOGY, 1962, 74 (04): : 387 - &
[5] GLYCOL METHACRYLATE IN LIGHT-MICROSCOPY - ROUTINE METHOD FOR EMBEDDING AND SECTIONING ANIMAL-TISSUES
COLE, MB
SYKES, SM
STAIN TECHNOLOGY, 1974, 49 (06): : 387 - 400
[6] GLYCOL METHACRYLATE EMBEDDING OF BONE AND CARTILAGE FOR LIGHT MICROSCOPIC STAINING
COLE, MB
JOURNAL OF MICROSCOPY, 1982, 127 (AUG) : 139 - 148
[7] LIGHT-MICROSCOPY OF ECTOMYCORRHIZAS USING GLYCOL METHACRYLATE
ALEXANDER, IJ
BIGG, WL
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1981, 77 (OCT): : 425 - 429
[8] GLYCOL METHACRYLATE EMBEDDING - AN IMPROVED PROCEDURE FOR LOCALIZATION OF SOME ENZYMES FOR OPTICAL MICROSCOPY
BHANDARI, NN
SACHDEVA, A
CURRENT SCIENCE, 1984, 53 (22): : 1186 - 1188
[9] DIETHYLENE GLYCOL DISTEARATE EMBEDDING + ULTRAMICROTOME SECTIONING FOR LIGHT MICROSCOPY
SALAZAR, H
STAIN TECHNOLOGY, 1964, 39 (01): : 13 - &
[10] GLYCOL METHACRYLATE EMBEDDING FOR LIGHT MICROSCOPY-I ENZYME-HISTOCHEMISTRY ON SEMITHIN SECTIONS OF UNDECALCIFIED MARROW CORES
ISLAM, A
HENDERSON, ES
JOURNAL OF CLINICAL PATHOLOGY, 1987, 40 (10) : 1194 - 1200

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