Simple DNA extraction of urine samples: Effects of storage temperature and storage time

被引：16

作者：

Ng, Huey Hian ^{[1
]}

Ang, Hwee Chen ^{[1
]}

Hoe, See Ying ^{[1
]}

Lim, Mae-Lynn ^{[1
]}

Tai, Hua Eng ^{[1
]}

Soh, Richard Choon Hock ^{[2
]}

Syn, Christopher Kiu-Choong ^{[1
]}

机构：

[1] Hlth Sci Author, Appl Sci Grp, DNA Profiling Lab, 3 Biopolis Dr, Singapore 138623, Singapore

[2] Minist Home Affairs, Cent Narcot Bur, 393 New Bridge Rd, Singapore 088763, Singapore

来源：

FORENSIC SCIENCE INTERNATIONAL | 2018年 / 287卷

关键词：

DNA extraction; Maxwell; 16; Urine sample; Storage condition;

D O I：

10.1016/j.forsciint.2018.03.035

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Urine samples are commonly analysed in cases with suspected illicit drug consumption. In events of alleged sample mishandling, urine sample source identification may be necessary. A simple DNA extraction procedure suitable for STR typing of urine samples was established on the Promega Maxwell (R) 16 paramagnetic silica bead platform. A small sample volume of 1.7 mL was used. Samples were stored at room temperature, 4 degrees C and -20 degrees C for 100 days to investigate the influence of storage temperature and time on extracted DNA quantity and success rate of STR typing. Samples stored at room temperature exhibited a faster decline in DNAyield with time and lower typing success rates as compared to those at 4 degrees C and -20 degrees C. This trend can likely be attributed to DNA degradation. In conclusion, this study presents a quick and effective DNA extraction protocol from a small urine volume stored for up to 100 days at 4 degrees C and -20 degrees C. (c) 2018 Elsevier B.V. All rights reserved.

引用

页码：36 / 39

页数：4

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