The hns Gene of Escherichia coli Is Transcriptionally Down-Regulated by (p)ppGpp

被引:6
|
作者
Brandi, Anna [1 ]
Giangrossi, Mara [1 ]
Fabbretti, Attilio [1 ]
Falconi, Maurizio [1 ]
机构
[1] Univ Camerino, Sch Biosci & Vet Med, I-62032 Camerino, Italy
关键词
hns; guanosine penta-tetra-phosphate; stringent response; transcriptional regulation; DNA-BINDING PROTEIN; NUCLEOID-ASSOCIATED PROTEIN; H-NS; RIBOSOMAL-RNA; STRINGENT RESPONSE; STRUCTURAL BASIS; PPGPP BINDING; PROMOTER; POLYMERASE; EXPRESSION;
D O I
10.3390/microorganisms8101558
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Second messenger nucleotides, such as guanosine penta- or tetra-phosphate, commonly referred to as (p)ppGpp, are powerful signaling molecules, used by all bacteria to fine-tune cellular metabolism in response to nutrient availability. Indeed, under nutritional starvation, accumulation of (p)ppGpp reduces cell growth, inhibits stable RNAs synthesis, and selectively up- or down- regulates the expression of a large number of genes. Here, we show that the E. coli hns promoter responds to intracellular level of (p)ppGpp. hns encodes the DNA binding protein H-NS, one of the major components of bacterial nucleoid. Currently, H-NS is viewed as a global regulator of transcription in an environment-dependent mode. Combining results from relA (ppGpp synthetase) and spoT (ppGpp synthetase/hydrolase) null mutants with those from an inducible plasmid encoded RelA system, we have found that hns expression is inversely correlated with the intracellular concentration of (p)ppGpp, particularly in exponential phase of growth. Furthermore, we have reproduced in an in vitro system the observed in vivo (p)ppGpp-mediated transcriptional repression of hns promoter. Electrophoretic mobility shift assays clearly demonstrated that this unusual nucleotide negatively affects the stability of RNA polymerase-hns promoter complex. Hence, these findings demonstrate that the hns promoter is subjected to an RNA polymerase-mediated down-regulation by increased intracellular levels of (p)ppGpp.
引用
收藏
页码:1 / 14
页数:13
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