Molecular Characterization of Brazilian Infectious Bursal Disease Virus Isolated from 1997 to 2005

被引：12

作者：

Fernandes, Maria Judite B. ^{[2
]}

Simoni, Isabela C. ^{[2
]}

Vogel, Maria Gabriela ^{[2
]}

Harakava, Ricardo ^{[2
]}

Rivas, Eliana B. ^{[2
]}

Oliveira, Maria Beatriz C. ^{[3
]}

Kanashiro, Ana M. I. ^{[2
]}

Tessari, Eliana N. C. ^{[2
]}

Gama, Nilce M. S. Q. ^{[2
]}

Arns, Clarice W. ^{[1
]}

机构：

[1] Univ Estadual Campinas, Campinas, SP, Brazil

[2] Inst Biol, Sao Paulo, Brazil

[3] Lohmann Anim Hlth, BR-05412001 Rua Joao Moura, SP, Brazil

来源：

AVIAN DISEASES | 2009年 / 53卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

infectious bursal disease virus; sequence analysis; VP2 hypervariable region; very virulent; Brazilian isolates; SEQUENCE-ANALYSIS; VARIABLE REGION; VIRULENT; IDENTIFICATION; POULTRY; STRAINS; DOMAIN;

D O I：

10.1637/8531-112808-Reg.1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleotide and phylogenetic analysis. All of them had the typical amino acid residues, and all clustered in a phylogenetic tree with the vvIBDV strains. Four amino acid substitutions, at positions D213N, G254D, S317R, and D323E, were common to 3 vv isolates, Br/03/DB, Br/03/CK, and Br/04/CR, and differed from other vv isolates and strains. These isolates came from the same locale, but were collected in different years, indicating that the vvIBDVs circulating on Brazilian farms are undergoing slight but continuous exchanges.

引用

页码：449 / 454

页数：6

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