FNDC5 relates to skeletal muscle IGF-I and mitochondrial function and gene expression in obese men with reduced growth hormone

被引：23

作者：

Srinivasa, Suman ^{[1
,2
]}

Suresh, Caroline ^{[1
,2
]}

Mottla, Jay ^{[3
]}

Hamarneh, Sulaiman R. ^{[2
,4
]}

Irazoqui, Javier E. ^{[2
,5
]}

Frontera, Walter ^{[6
,7
,8
]}

Torriani, Martin ^{[2
,9
]}

Stanley, Takara ^{[1
,2
,10
]}

Makimura, Hideo ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Program Nutr Metab, 55 Fruit St,LON207, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Boston, MA USA

[3] Georgetown Univ, Sch Med, Washington, DC USA

[4] Massachusetts Gen Hosp, Dept Surg, Boston, MA 02114 USA

[5] Massachusetts Gen Hosp, Ctr Study Inflammatory Bowel Dis, Lab Comparat Immunol, Boston, MA 02114 USA

[6] Vanderbilt Univ, Med Ctr, Dept Phys Med & Rehabil, Nashville, TN USA

[7] Harvard Univ, Sch Med, Dept Phys Med & Rehabil, Spaulding Rehabil Hosp, Boston, MA USA

[8] Univ Puerto Rico, Sch Med, Dept Physiol, San Juan, PR USA

[9] Massachusetts Gen Hosp, Div Musculoskeletal Imaging & Intervent, Boston, MA 02114 USA

[10] Massachusetts Gen Hosp, Dept Pediat, Boston, MA 02114 USA

来源：

GROWTH HORMONE & IGF RESEARCH | 2016年 / 26卷

关键词：

lrisin; FNDC5; Growth hormone; IGF-I; Skeletal muscle; Mitochondrial; Obesity; MESSENGER-RNA EXPRESSION; PHOSPHOCREATINE RECOVERY; IRISIN LEVELS; EXERCISE; SERUM; MYOKINE; HUMANS; BIOPSY; PLASMA; YOUNG;

D O I：

10.1016/j.ghir.2015.12.008

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective: To investigate the relationship of skeletal muscle FNDC5 mRNA expression and circulating irisin to the GH/IGF-I axis and to skeletal muscle mitochondrial function and mitochondria-related gene expression in obese men. Design: Fifteen abdominally obese men with reduced growth hormone received 12 weeks of recombinant human GH (rhGH). Before and after treatment, they underwent P-31-magnetic resonance spectroscopy to evaluate phosphocreatine (PCr) recovery as a measure of mitochondrial function and skeletal muscle biopsy to assess expression of mitochondrial-related genes. Serum irisin and IGF-I and skeletal muscle FNDC5 and IGF-I mRNA were measured. Results: At baseline, skeletal muscle FNDC5 mRNA was significantly and positively associated with IGF-I mRNA (rho = 0.81, P = 0.005) and rate of PCr recovery (rho = 0.79, P = 0.006). Similar relationships of circulating irisin to IGF-I mRNA (rho = 0.63, P = 0.05) and rate of PCr recovery (rho = 0.48, P = 0.08) were demonstrated, but were not as robust as those with muscle FNDC5 expression. Both serum irisin and skeletal muscle FNDC5 mRNA were significantly associated with PPAR gamma (rho = 0.73, P = 0.02 and rho = 0.85, P = 0.002), respectively. In addition, FNDC5 mRNA was correlated with skeletal muscle PGC-1 alpha (rho = 0.68, P = 0.03), NRF1 (rho = 0.66, P = 0.04) and TFAM (rho = 0.79, P = 0.007) mRNA. Neither serum irisin nor muscle mRNA expression of FNDC5 changed with rhGH treatment. Conclusion: These novel data in skeletal muscle demonstrate that local expression of FNDC5 is associated with mRNA expression of IGF-I and mitochondrial function and mitochondria-related gene expression in obese subjects with reduced growth hormone and suggest a potential role for FNDC5 acting locally in muscle in a low GH state. Further studies are needed to clarify the relationship between the GH/IGF-I axis and irisin. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：36 / 41

页数：6

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