Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray

被引：28
|
作者：
Lan, Y.
Xu, X.
Yang, F.
Zhang, X. [1 ]
机构：
[1] SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China
[2] Xiamen Univ, Coll Med, Biomed Engn Res Ctr, Xiamen 361005, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2006年 / 151卷 / 09期
关键词：
D O I：
10.1007/s00705-006-0751-x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In an attempt to obtain the transcriptional profile of shrimp white spot syndrome virus (WSSV) genes, DNA microarray analysis was performed using amplified DNA fragments of the WSSV genome that covered most of the presumptive open reading frames (ORFs). Total RNAs were extracted from WSSV-infected crayfish (Cambarus clarkii) and reverse transcribed into cDNA and labeled with P-32-dATP. The DNA microarray detected 81.1% of the ORFs, and 47 of these were transcribed at 6 h post infection, suggesting they may be early genes. Some early genes were further confirmed by temporal analyses of gene transcription. Results in this study indicated the effectiveness of DNA microarray for discovering early viral genes.
引用
收藏
页码：1723 / 1733
页数：11
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