Comparison of the homologous SfeI and LlaBI restriction-modification systems

被引:0
|
作者
Okhapkina, SS [1 ]
Netesova, NA
Golikova, LN
Seregina, EV
Sosnovtsev, SV
Abdurashitov, MA
Degtyarev, SK
机构
[1] Vektor State Res Ctr Virol & Biotechnol, Inst Mol Biol, Koltsov 633159, Novosibirsk, Russia
[2] Russian Acad Med Sci, Siberian Div, Inst Mol Biol & Biophys, Novosibirsk 630117, Russia
关键词
Streptococcus faecalis; restriction-modification system; gene cloning; an-Lino acid sequence alignment; horizontal transfer;
D O I
10.1023/A:1016094926458
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'. A substantial difference was that SfeI RMS operon had an additional 198-bp segment in the methylase gene and a larger gene for the putative control protein. No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon.
引用
收藏
页码:333 / 337
页数:5
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