β-N-ACETYLGLUCOSAMINIDASE PRODUCTION BY Lecanicillium (Verticillium) lecanii ATCC 26854 BY SOLID-STATE FERMENTATION UTILIZING SHRIMP SHELL

被引：0
|
作者：
Barranco Florido, Esteban [1 ,2 ,3 ]
Bustamante Camilo, Patricia [2 ,3 ]
Mayorga-Reyes, Lino [2 ,4 ]
Gonzalez Cervantes, Rina M. [2 ,3 ]
Martinez Cruz, Patricia [2 ,5 ]
Azaola, Alejandro [3 ]
机构：
[1] UAM, Unidad Xochimilco, Dept Sistemas Biol, Coyoacan 04960, Mexico
[2] UAM, Xochimilco, Mexico
[3] UAM, Mexico City, DF, Mexico
[4] IPN, CINVESTAV, Mexico City 07738, DF, Mexico
[5] Univ Nacl Autonoma Mexico, Mexico City 04510, DF, Mexico
来源：
INTERCIENCIA | 2009年 / 34卷 / 05期
关键词：
Antagonism; beta-N-acetylglucosaminidase; Lecanicillium lecanii; Oidium ssp; Solid State Fermentation; FUNGUS METARHIZIUM-ANISOPLIAE; ENTOMOPATHOGENIC FUNGI; TRICHODERMA-HARZIANUM; CHITINOLYTIC ENZYMES; CHITINASE; ENDOCHITINASE; CHITOSAN; DELIVERY; SYSTEMS; SILAGE;
D O I：
暂无
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
Lecanicillium (Verticillium) lecanii produced chitinases using shrimp shell as inducer Maximum production of beta-N-acetylglucosaminidase was measured at 80h. Enzyme stability was obtained at temperatures ranging from 30 to 40 degrees C and maxi. mum activity at 50 degrees C, pH 6.0. Enzyme activity increased with Ba2+, Co2+, Fe3+ and Zn2+. Bioassays against the phytopathogenic fungus Oidium spp. showed mycelial and germi-nation inhibition. SDS-PAGE electrophoresis of the partially purified extract revealed four bands of 70, 58, 45 and 31kDa and this extract showed activity of beta-N-acetylglucosaminidase through zymogram analysis. Chitinases produced by L. lecanii are potentially useful against phytopathogenic fungi, insects and chitosan bioconversions.
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收藏
页码：356 / 360
页数：5
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