β-N-ACETYLGLUCOSAMINIDASE PRODUCTION BY Lecanicillium (Verticillium) lecanii ATCC 26854 BY SOLID-STATE FERMENTATION UTILIZING SHRIMP SHELL

被引：0

作者：

Barranco Florido, Esteban ^{[1
,2
,3
]}

Bustamante Camilo, Patricia ^{[2
,3
]}

Mayorga-Reyes, Lino ^{[2
,4
]}

Gonzalez Cervantes, Rina M. ^{[2
,3
]}

Martinez Cruz, Patricia ^{[2
,5
]}

Azaola, Alejandro ^{[3
]}

机构：

[1] UAM, Unidad Xochimilco, Dept Sistemas Biol, Coyoacan 04960, Mexico

[2] UAM, Xochimilco, Mexico

[3] UAM, Mexico City, DF, Mexico

[4] IPN, CINVESTAV, Mexico City 07738, DF, Mexico

[5] Univ Nacl Autonoma Mexico, Mexico City 04510, DF, Mexico

来源：

INTERCIENCIA | 2009年 / 34卷 / 05期

关键词：

Antagonism; beta-N-acetylglucosaminidase; Lecanicillium lecanii; Oidium ssp; Solid State Fermentation; FUNGUS METARHIZIUM-ANISOPLIAE; ENTOMOPATHOGENIC FUNGI; TRICHODERMA-HARZIANUM; CHITINOLYTIC ENZYMES; CHITINASE; ENDOCHITINASE; CHITOSAN; DELIVERY; SYSTEMS; SILAGE;

D O I：

暂无

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Lecanicillium (Verticillium) lecanii produced chitinases using shrimp shell as inducer Maximum production of beta-N-acetylglucosaminidase was measured at 80h. Enzyme stability was obtained at temperatures ranging from 30 to 40 degrees C and maxi. mum activity at 50 degrees C, pH 6.0. Enzyme activity increased with Ba2+, Co2+, Fe3+ and Zn2+. Bioassays against the phytopathogenic fungus Oidium spp. showed mycelial and germi-nation inhibition. SDS-PAGE electrophoresis of the partially purified extract revealed four bands of 70, 58, 45 and 31kDa and this extract showed activity of beta-N-acetylglucosaminidase through zymogram analysis. Chitinases produced by L. lecanii are potentially useful against phytopathogenic fungi, insects and chitosan bioconversions.

引用

页码：356 / 360

页数：5

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