Interlaboratory variability in fluorescence in situ hybridization analysis

被引:13
|
作者
Moore, DH
Epstein, L
Reeder, J
Wheeless, L
Waldman, FM
Aamodt, R
CordonCardo, C
Fradet, Y
Grossman, B
Hemstreet, G
机构
[1] NCI,BETHESDA,MD 20892
[2] MEM HOSP,NEW YORK,NY
[3] UNIV LAVAL,QUEBEC CITY,PQ,CANADA
[4] ANDERSON MED CTR,HOUSTON,TX
[5] UNIV OKLAHOMA,MED CTR,OKLAHOMA CITY,OK
[6] UNIV ROCHESTER,ROCHESTER,MI
来源
CYTOMETRY | 1996年 / 25卷 / 02期
关键词
fluorescence in situ hybridization; variability; bladder cancer; chi(2); multinomial distribution;
D O I
10.1002/cyto.990250202
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reliable interpretation of fluorescence in situ hybridization (FISH) data, especially data that have been generated in more than one laboratory, requires knowledge of the sources of variability inherent in FISH analysis, Possible sources of variation may derive from differences in sample preparation, probes used, intrasample heterogeneity, hybridization protocols, counting criteria within and between scorers, fluorescence microscopes, and filters. This study characterized the relative weight of some of these factors in order to determine the degree to which FISH results are comparable between laboratories, We used a hierarchical partitioned chi(2) analysis to measure sources of variation. We found that replicate counts varied no more than expected based on counting statistics (i.e., multinomial variation), However, with replicate hybridizations done in two separate laboratories, the variability increased significantly, Thus, care must be taken when interpreting FISH data that are derived from more than one institution, Previously agreed upon counting criteria as well as standardized FISH hybridization protocols may decrease this variability. (C) 1996 Wiley-Liss, Inc.
引用
收藏
页码:125 / 132
页数:8
相关论文
共 50 条
  • [41] Oncogene amplification in medulloblastoma:: analysis of a case by comparative genomic hybridization and fluorescence in situ hybridization
    Jay, V
    Squire, J
    Bayani, J
    Alkhani, AM
    Rutka, JT
    Zielenska, M
    PATHOLOGY, 1999, 31 (04) : 337 - 344
  • [42] Array Comparative Genomic Hybridization and Fluorescence In Situ Hybridization Analysis of Endometrial Carcinoma Specimens
    Voss, J. S.
    Kipp, B. R.
    Peterson, L. M.
    Medeiros, F.
    Campion, M. B.
    Pestova, E.
    Jacobson, K. B.
    Legator, M. S.
    Clayton, A. C.
    Halling, K. C.
    LABORATORY INVESTIGATION, 2011, 91 : 269A - 269A
  • [43] VARIABILITY IN RDNA LOCI IN THE GENUS ORYZA DETECTED THROUGH FLUORESCENCE IN-SITU HYBRIDIZATION
    FUKUI, K
    OHMIDO, N
    KHUSH, GS
    THEORETICAL AND APPLIED GENETICS, 1994, 87 (08) : 893 - 899
  • [44] In Situ Genetic Analysis of Hydatidiform Moles by Polymorphic Deletion Probe Fluorescence In Situ Hybridization
    Chiang, S.
    Fazlollahi, L.
    Nguyen, A.
    Roberts, D. J.
    Iafrate, A. J.
    MODERN PATHOLOGY, 2010, 23 : 237A - 237A
  • [45] In Situ Genetic Analysis of Hydatidiform Moles by Polymorphic Deletion Probe Fluorescence In Situ Hybridization
    Chiang, S.
    Fazlollahi, L.
    Nguyen, A.
    Roberts, D. J.
    Iafrate, A. J.
    LABORATORY INVESTIGATION, 2010, 90 : 237A - 237A
  • [46] Fluorescence in situ hybridization: Uses and limitations
    Gozzetti, A
    Le Beau, MM
    SEMINARS IN HEMATOLOGY, 2000, 37 (04) : 320 - 333
  • [47] Fluorescence in situ hybridization of freshwater fungi
    Baschien, C
    Manz, W
    Neu, TR
    Szewzyk, U
    INTERNATIONAL REVIEW OF HYDROBIOLOGY, 2001, 86 (4-5) : 371 - 381
  • [48] Progresses and applications of fluorescence in situ hybridization
    Wang, L
    Ning, SB
    Song, YC
    Lü, YT
    ACTA BOTANICA SINICA, 2000, 42 (11): : 1101 - 1107
  • [49] Fluorescence in situ hybridization in diagnostic cytolog
    Halling, Kevin C.
    Kipp, Benjamin R.
    HUMAN PATHOLOGY, 2007, 38 (08) : 1137 - 1144
  • [50] Fluorescence in situ hybridization protocol for cardiomyocytes
    Yao, Zehao
    Bai, Lina
    Nie, Yu
    JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2025, 201 : 44 - 51