Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum

被引：7
|
作者：
Su, Zengping [1 ]
Wang, Fengqin [1 ]
Xie, Yaohuan [1 ]
Xie, Hui [1 ]
Mao, Guotao [1 ]
Zhang, Hongsen [1 ]
Song, Andong [1 ]
Zhang, Zhanying [2 ,3 ]
机构：
[1] Henan Agr Univ, Coll Life Sci, Minist Agr, Key Lab Agr Microbial Enzyme Engn, 63 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China
[2] Queensland Univ Technol, Ctr Agr & Bioecon, Inst Future Environm, Brisbane, Qld 4000, Australia
[3] Queensland Univ Technol, Sci & Engn Fac, Sch Mech Med & Proc Engn, Brisbane, Qld 4000, Australia
来源：
SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期
基金：
中国国家自然科学基金;
关键词：
BIOBUTANOL PRODUCTION; ETHANOL PRODUCTION; PROTEOMIC ANALYSIS; FORMIC-ACID; FERMENTATION; BEIJERINCKII; DETOXIFICATION; SACCHAROPERBUTYLACETONICUM; SUPPLEMENTATION; SWITCHGRASS;
D O I：
10.1038/s41598-020-74899-9
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study, the role of CaCO3 in n-butanol production was further investigated using corn straw hydrolysate (CSH) media by Clostridium acetobutylicum CICC 8016. CaCO3 addition stimulated sugars utilization and butanol production. Further study showed that calcium salts addition to CSH media led to the increase in Ca2+ concentration both intracellularly and extracellularly. Interestingly, without calcium salts addition, intracellular Ca2+ concentration in the synthetic P2 medium was much higher than that in the CSH medium despite the lower extracellular Ca2+ concentrations in the P2 medium. These results indicated that without additional calcium salts, Ca2+ uptake by C. acetobutylicum CICC 8016 in the CSH medium may be inhibited by non-sugar biomass degradation compounds, such as furans, phenolics and organic acids. Comparative proteomics analysis results showed that most enzymes involved in glycolysis, redox balance and amino acids metabolism were up-regulated with CaCO3 addition. This study provides further insights into the role of CaCO3 in n-butanol production using real biomass hydrolysate.
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页数：10
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