Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism

被引：11

作者：

Parra, Elena ^{[1
]}

Segura, Ferran ^{[1
]}

Tijero, Jessica ^{[1
]}

Pons, Imma ^{[1
]}

Nogueras, Maria-Mercedes ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Corp Sanitaria Parc Tauli, Inst Invest Innovacio Sanitaria Parc Tauli 1, Infect Dis Dept, Parc Taruli 1, Barcelona 08208, Spain

来源：

MOLECULAR AND CELLULAR PROBES | 2017年 / 32卷

关键词：

Bartonella; Real-time PCR; Optimization; Zoonoses; CAT-SCRATCH DISEASE; INFECTIOUS-DISEASE; DIFFERENTIATION; ASSAY; HENSELAE; IDENTIFICATION; GRAHAMII; GENE; DNA;

D O I：

10.1016/j.mcp.2016.10.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction. (C) 2016 Published by Elsevier Ltd.

引用

页码：55 / 59

页数：5

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