Diacylglycerol kinase α enhances protein kinase Cζ-dependent phosphorylation at Ser311 of p65/RelA subunit of nuclear factor-κB

被引:31
|
作者
Kai, Masahiro [2 ]
Yasuda, Satoshi [2 ]
Imai, Shin-ichi [2 ]
Toyota, Minoru [2 ]
Kanoh, Hideo [2 ]
Sakane, Fumio [1 ]
机构
[1] Chiba Univ, Dept Chem, Grad Sch Sci, Inage Ku, Chiba 2638522, Japan
[2] Sapporo Med Univ, Sch Med, Dept Biochem, Sapporo, Hokkaido, Japan
关键词
Diacylglycerol kinase; Nuclear factor-kappa B; Tumor necrosis factor-alpha; Phosphorylation; Protein kinase C zeta; ACTIVATION; CELLS; P65;
D O I
10.1016/j.febslet.2009.09.017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently reported that diacylglycerol kinase (DGK) alpha enhanced tumor necrosis factor-alpha (TNF-alpha)-induced activation of nuclear factor-kappa B (NF-kappa B). However, the signaling pathway between DGK alpha and NF-kappa B remains unclear. Here, we found that small interfering RNA-mediated knockdown of DGK alpha strongly attenuated protein kinase C (PKC) zeta-dependent phosphorylation of a large subunit of NF-kappa B, p65/RelA, at Ser311 but not PKC zeta-independent phosphorylation at Ser468 or Ser536. Moreover, knockdown and overexpression of PKC zeta suppressed and synergistically enhanced DGK alpha-mediated NF-kappa B activation, respectively. These results strongly suggest that DGKa positively regulates TNF-alpha-dependent NF-kappa B activation via the PKC zeta-mediated Ser311 phosphorylation of p65/RelA. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:3265 / 3268
页数:4
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