Chromatic two-photon excitation fluorescence imaging

被引：8
|
作者：
Xu, Q. [1 ]
Shi, K. [1 ]
Yin, S. [1 ]
Liu, Z. [1 ]
机构：
[1] Penn State Univ, Dept Elect Engn, University Pk, PA 16802 USA
来源：
JOURNAL OF MICROSCOPY | 2009年 / 235卷 / 01期
基金：
美国国家科学基金会;
关键词：
Chromatic axial scanning; two-photon fluorescence microscopy; CONFOCAL MICROSCOPY; MULTIPHOTON MICROSCOPY; FEMTOSECOND; LENSES; LASER;
D O I：
10.1111/j.1365-2818.2009.03183.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
P>We report on a chromatic axial scanning method for two-photon excitation fluorescence imaging. Effective axial scanning is achieved by incorporating a Fresnel lens in the system, which has large chromatic aberration and can therefore focus the excitation beam to different axial positions depending on its wavelength. We experimentally demonstrated this technique and used it to image the cross-section of fluorescent microspheres.
引用
收藏
页码：79 / 83
页数：5
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