Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

被引:54
|
作者
Iempridee, Tawin [1 ]
机构
[1] Natl Sci & Technol Dev Agcy, Natl Nanotechnol Ctr NANOTEC, Thailand Sci Pk, Pathum Thani 12120, Thailand
关键词
lncRNA; H19; cervical cancer; extracellular vesicles; transforming growth factor beta; hypoxia; TUMOR-SUPPRESSOR; POOR-PROGNOSIS; METASTASIS; EXPRESSION; LNCRNA; CONTRIBUTES; MIGRATION; MALAT-1; HYPOXIA; WOMEN;
D O I
10.1177/1535370216670542
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl2 could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific and independent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy.
引用
收藏
页码:184 / 193
页数:10
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