Effects of phospholipids on growth of Chinese hamster ovary cells in serum-free media

被引:9
|
作者
Sakai, K
Matsunaga, T
Yamaji, H
Fukuda, H
机构
[1] Kobe Univ, Div Mol Sci, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Dept Sci & Chem Engn, Fac Engn, Nada Ku, Kobe, Hyogo 6578501, Japan
关键词
phosphatidic acid; lysophosphatidic acid; CHO cells; serum-free medium; MTT assay;
D O I
10.1016/S1389-1723(00)80014-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effects of phosphatidic acid (PA) and lysophosphatidic acid (LPA) dispersed in serum-free media on the growth of Chinese hamster ovary (CHO) cells were investigated. After cells were incubated in serum-free media supplemented with PA or LPA for 3 d, the cellular growth was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Supplementing PA from egg yolk lecithin to basal synthetic media such as alpha-MEM (minimum essential medium, alpha modification) markedly promoted the growth of CHO cells. PA from egg yolk lecithin also enhanced the cell growth in a serum-free medium containing insulin and transferrin. When PA analogues with different acyl chains of C14-18 were compared, PA with unsaturated acyl chains, dioleoyl-PA (C18 : 1), was most effective far stimulating the growth of CHO cells. Similar results were obtained when LPA was examined. These results suggest that PA and LPA, especially those with unsaturated acyl chains, are promising growth-promoting supplements for use as constituents in a low-protein serum-free medium.
引用
收藏
页码:306 / 309
页数:4
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