The solution structure of the S1 RNA binding domain: A member of an ancient nucleic acid-binding fold

被引:360
|
作者
Bycroft, M [1 ]
Hubbard, TJP [1 ]
Proctor, M [1 ]
Freund, SMV [1 ]
Murzin, AG [1 ]
机构
[1] UNIV CAMBRIDGE, CTR MRC, CAMBRIDGE CB2 2QH, ENGLAND
基金
英国医学研究理事会;
关键词
D O I
10.1016/S0092-8674(00)81844-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The S1 domain, originally identified in ribosomal protein S1, is found in a large number of RNA-associated proteins. The structure of the S1 RNA-binding domain from the E. coli polynucleotide phosphorylase has been determined using NMR methods and consists of a five-stranded antiparallel beta barrel. Conserved residues on one face of the barrel and adjacent loops form the putative RNA-binding site. The structure of the S1 domain is very similar to that of cold shock protein, suggesting that they are both derived from an ancient nucleic acid-binding protein. Enhanced sequence searches reveal hitherto unidentified S1 domains in RNase E, RNase II, NusA, EMB-5, and other proteins.
引用
收藏
页码:235 / 242
页数:8
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