Copper-Free Click Chemistry Assisted Antibodies Immobilization for Immunosensing Of IL-10 Cytokine

被引：1

作者：

Hilali, Nazha ^{[1
,2
]}

Abdullahi, Musbahu Aminu ^{[2
]}

Bellagambi, Francesca G. ^{[2
]}

Hangouet, Marie ^{[2
]}

Elaissari, Abdelhamid ^{[2
]}

Jaffrezic-Renult, Nicole ^{[2
]}

Bausells, Joan ^{[3
]}

Mohammadi, Hasna ^{[1
]}

Amine, Aziz ^{[1
]}

Zine, Nadia ^{[2
]}

Errachid, Abdelhamid ^{[2
]}

机构：

[1] Hassan II Univ Casablanca, Fac Sci & Tech, Lab Proc Engn & Environm, BP 146, Mohammadia 28806, Morocco

[2] Univ Lyon1, Inst Analyt Sci, UMR 5280, 5 Rue Doua, F-69100 Villeurbanne, France

[3] Ctr Natl Microelect IMB CSIC, Campus UAB, Barcelona 08193, Spain

来源：

PROCEEDINGS OF INTERNATIONAL WORKSHOP ON IMPEDANCE SPECTROSCOPY (IWIS 2021) | 2021年

基金：

欧盟地平线“2020”;

关键词：

Copper-free click chemistry; Immunosensor; Micro-contact printing; Interleukin-10; Heart failure monitoring; ELECTROCHEMICAL BIOSENSOR PLATFORM; MONOLAYERS;

D O I：

10.1109/IWIS54661.2021.9711762

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Copper-free variant of click chemistry has been successfully implemented to develop a new electrochemical immunosensor for cytokine interleukin-10 (IL-10) detection in saliva for heart failure (HF) monitoring. The approach involved the binding of dibenzocyclooctyne-amine (DBCO-NH2) onto the azide-thiol modified gold microelectrodes. The use of dibenzocyclooctynes decreased the activation energy for the cycloaddition click reaction, enabling its realization without the need for copper catalysis and with an efficiency greater than that of the copper catalysed ligation. Then, the biofunctionalization was performed through incubating the prepared sensor in the capture antibody of IL-10 (Anti-IL-10). The conjugation was formed via carbodiimide cross-linking and cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used for characterizing step-by-step the modification of the gold microelectrode.

引用

页码：20 / 25

页数：6

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