AN EWOD DROPLET MICROFLUIDIC CHIP WITH INTEGRATED LOCAL TEMPERATURE CONTROL FOR MULTIPLEX PROTEOMICS

被引：4

作者：

Nelson, Wyatt ^{[1
]}

Peng, Ivory ^{[2
]}

Loo, Joseph A. ^{[2
]}

Garrell, Robin L. ^{[2
]}

Kim, Chang-Jin C. J. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Mech & Aerosp Engn Dept, Los Angeles, CA 90024 USA

[2] UCLA, Dept Chem & Biochem, Los Angeles, CA USA

来源：

IEEE 22ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2009) | 2009年

关键词：

AMPLIFICATION;

D O I：

10.1109/MEMSYS.2009.4805373

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present a microfluidic chip for multiplex thermal processing of discrete micro-and nano-liter sample droplets driven by electrowetting-on-dielectric (EWOD). The system features local temperature control integrated on chip in addition to the basic fluidic functionalities previously reported for EWOD devices [1]. Multi-functioning indium-tin-oxide (ITO) electrodes serve as optically transparent resistive heaters, temperature sensors, and EWOD actuation pads. With a 1 mu L sample, heaters reach programmed set points in a few seconds and stabilize to +/- 1 degrees C. We demonstrate automated on-chip proteomics sample processing and direct characterization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The results, showing increased insulin disulfide reduction efficiency with heater temperatures from 22 to 130 degrees C, highlight the efficacy of the chip.

引用

页码：280 / 283

页数：4

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