Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

被引:39
|
作者
Lei, Xianlu [1 ,2 ]
Xu, Liguang [1 ,2 ]
Song, Shanshan [1 ,2 ]
Liu, Liqiang [1 ,2 ]
Kuang, Hua [1 ,2 ]
机构
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi, Jiangsu, Peoples R China
关键词
Florfenicol; ic-ELISA; immunochromatographic strip assay; monoclonal antibody; thiamphenicol; ENZYME-LINKED-IMMUNOSORBENT; MONOCLONAL-ANTIBODY; LIQUID-CHROMATOGRAPHY; RAPID DETECTION; DRINKING-WATER; MILK SAMPLES; CHLORAMPHENICOL; AMINE; RACTOPAMINE; FEED;
D O I
10.1080/09540105.2017.1371114
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples?. ? ? The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2ng/mL for FF and 0.27ng/mL for TAP, with the working range of 0.05-0.77 and 0.05-1.42ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.
引用
收藏
页码:254 / 266
页数:13
相关论文
共 50 条
  • [21] Development of IC-ELISA for detection of organophosphorus pesticides in water
    Tang, Jianshe
    Zhang, Min
    Cheng, Guohua
    Li, Ang
    Lu, Yitong
    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2008, 43 (08) : 707 - 712
  • [22] Development of an Immunochromatographic Strip for the Rapid and Ultrasensitive Detection of Gamithromycin
    Lu, Qianqian
    Xu, Xinxin
    Song, Shanshan
    Wu, Aihong
    Liu, Liqiang
    Kuang, Hua
    Xu, Chuanlai
    FOOD ANALYTICAL METHODS, 2022, 15 (03) : 598 - 606
  • [23] Development of an Immunochromatographic Strip for the Rapid and Ultrasensitive Detection of Gamithromycin
    Qianqian Lu
    Xinxin Xu
    Shanshan Song
    Aihong Wu
    Liqiang Liu
    Hua Kuang
    Chuanlai Xu
    Food Analytical Methods, 2022, 15 : 598 - 606
  • [24] Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips
    Shen, Xinyi
    Liu, Liqiang
    Xu, Liguang
    Ma, Wei
    Wu, Xiaoling
    Cui, Gang
    Kuang, Hua
    FOOD AND AGRICULTURAL IMMUNOLOGY, 2019, 30 (01) : 913 - 923
  • [25] Development of ELISA and immunochromatographic assay for the detection of neomycin
    Jin, Y
    Jang, JW
    Lee, MH
    Han, CH
    CLINICA CHIMICA ACTA, 2006, 364 (1-2) : 260 - 266
  • [26] Development of ELISA and immunochromatographic assay for the detection of gentamicin
    Jin, Y
    Jang, JW
    Han, CH
    Lee, MH
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (20) : 7639 - 7643
  • [27] Development of ic-ELISA and colloidal cold-based immunochromatographic assay for red 2G detection in fruit drinks, red wine, and yoghurts
    Li, Xiaoling
    Xu, Xinxin
    Guo, Lingling
    Song, Shanshan
    Liu, Liqiang
    Zhu, Yingyue
    Kuang, Hua
    Xu, Chuanlai
    Xu, Liguang
    FOOD BIOSCIENCE, 2023, 52
  • [28] Development of an Ultrasensitive Immunochromatographic Assay (ICA) Strip for the Rapid Detection of Phenylethanolamine A in Urine and Pork Samples
    Li Junhua
    Li Chunsheng
    Wu Meng
    Zhang Yan
    Ma Xiaofei
    Cheng Hua
    Yan Jinghui
    JOURNAL OF FOOD SCIENCE, 2015, 80 (04) : T894 - T899
  • [29] Determination of Organophosphorus Pesticides in Vegetables and Fruit by an Indirect Competitive Enzyme-Linked Immunosorbent Assay (ic-ELISA) and a Lateral-Flow Immunochromatographic (LFIC) Strip Assay
    Yue, Yadong
    Chen, Jiangyan
    Zhang, Mingjiang
    Yin, Yingai
    Dong, Yiyang
    ANALYTICAL LETTERS, 2022, 55 (11) : 1701 - 1718
  • [30] Development of an ic-ELISA and a TRFICA for the detection of fipronil with a new hapten design
    Liu, Junwei
    Wu, Yuqing
    Liu, Chenchen
    Cai, Yan
    Zhang, Rui
    Lu, Yang
    JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2024, 18 (04) : 2573 - 2586