RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

被引：33

作者：

Lee, Jeong-Soo ^{[1
,2
]}

Kwak, Su-Jin ^{[1
]}

Kim, Jungeun ^{[3
,4
]}

Kim, Ae-Kyeong ^{[1
]}

Noh, Hae Min ^{[1
]}

Kim, Jin-Soo ^{[3
,4
,5
]}

Yu, Kweon ^{[1
,2
]}

机构：

[1] KRIBB, Neurophysiol Res Grp, Bionano Ctr, Taejon 305806, South Korea

[2] KUST, Funct Genom Dept, Taejon 305333, South Korea

[3] Seoul Natl Univ, Natl Creat Res Initiat Ctr Genome Engn, Seoul 151747, South Korea

[4] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

[5] Inst for Basic Sci, Ctr Genome Engn, Taejon, South Korea

来源：

G3-GENES GENOMES GENETICS | 2014年 / 4卷 / 07期

关键词：

Cas9; protein; RGEN; Drosophila; GENE KNOCKOUT; HUMAN-CELLS; ENDONUCLEASES; NUCLEASES; CRISPR/CAS9; MICE;

D O I：

10.1534/g3.114.012179

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.

引用

页码：1291 / 1295

页数：5

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