Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans

被引:66
|
作者
Rangsinth, Panthakarn [1 ,5 ]
Prasansuklab, Anchalee [2 ]
Duangjan, Chatrawee [1 ,5 ]
Gu, Xiaojie [3 ,5 ]
Meemon, Krai [4 ]
Wink, Michael [5 ]
Tencomnao, Tewin [6 ]
机构
[1] Chulalongkorn Univ, Fac Allied Hlth Sci, Dept Clin Chem, Grad Program Clin Biochem & Mol Med, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Coll Publ Hlth Sci, Bangkok 10330, Thailand
[3] Dalian Jiaotong Univ, Sch Environm & Chem Engn, Dept Biotechnol, Dalian 116028, Peoples R China
[4] Mahidol Univ, Fac Sci, Dept Anat, Bangkok 10400, Thailand
[5] Heidelberg Univ, Inst Pharm & Mol Biotechnol, Neuenheimer Feld 364, D-69120 Heidelberg, Germany
[6] Chulalongkorn Univ, Fac Allied Hlth Sci, Dept Clin Chem, Age Related Inflammat & Degenerat Res Unit, Bangkok 10330, Thailand
来源
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2019年 / 19卷 / 1期
关键词
Caesalpinia mimosoides; Caenorhabditis elegans; Antioxidant; Oxidative stress; Aging; DAF-16; ANTIOXIDANT ACTIVITY; REACTIVE OXYGEN; DYSFUNCTION; GENOMICS; BIOLOGY; SKN-1; ROS;
D O I
10.1186/s12906-019-2578-5
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
BackgroundCaesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.MethodsC. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.ResultsA leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.ConclusionsThe results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
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页数:13
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