Estimation of valacyclovir in tablets and human serum by RP-HPLC method

A simple, rapid, sensitive and precise revenge phase-high performance liquid chromatographic (BPLC) method has been developed for the estimation of valacyclovir in., tablets and human serum. In this method, RP-C-18 column (150x4.6 mm I.D., 5 mu m particle size) with mobile phase consisting of acetonitrile and 0.03 M phosphate buffer pH 2.99 in the ratio of 50: 50 (v v) misocratic mode was used. The detection wavelength is 230 nm and the flow rate is 0.9 mL/min. In the range of 0.05-8 mu g/mL, the linearity of valacyclovir shows a con-elation coefficient of 0.9999. The mean recovery of the drug from the spiked serum solution Was found to be 97.82%. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters. The high percentage of recovery indicates that there is no interference from the other serum contents.