7 Tesla MRI Followed by Histological 3D Reconstructions in Whole-Brain Specimens

被引:23
|
作者
Alkemade, Anneke [1 ]
Pine, Kerrin [2 ]
Kirilina, Evgeniya [2 ,3 ]
Keuken, Max C. [1 ]
Mulder, Martijn J. [1 ,4 ]
Balesar, Rawien [1 ,5 ]
Groot, Josephine M. [1 ]
Bleys, Ronald L. A. W. [6 ]
Trampel, Robert [2 ]
Weiskopf, Nikolaus [2 ]
Herrler, Andreas [7 ]
Moller, Harald E. [8 ]
Bazin, Pierre-Louis [1 ,2 ,9 ]
Forstmann, Birte U. [1 ]
机构
[1] Univ Amsterdam, Integrat Model Based Neurosci Res Unit, Amsterdam, Netherlands
[2] Max Planck Inst Human Cognit & Brain Sci, Dept Neurophys, Leipzig, Germany
[3] Free Univ Berlin, Neurocomputat & Neuroimaging Unit, Dept Psychol & Educ Sci, Berlin, Germany
[4] Univ Utrecht, Dept Expt Psychol, Utrecht, Netherlands
[5] Inst Royal Netherlands Acad Arts & Sci, Netherlands Inst Neurosci, Amsterdam, Netherlands
[6] Univ Utrecht, Univ Med Ctr Utrecht, Dept Anat, Utrecht, Netherlands
[7] Maastricht Univ, Dept Anat & Embryol, Maastricht, Netherlands
[8] Max Planck Inst Human Cognit & Brain Sci, NMR Methods & Dev Grp, Leipzig, Germany
[9] Max Planck Inst Human Cognit & Brain Sci, Dept Neurol, Leipzig, Germany
来源
FRONTIERS IN NEUROANATOMY | 2020年 / 14卷
基金
欧洲研究理事会;
关键词
post mortemhuman brain; ultra-high field MRI; whole brain imaging; histology; formalin fixation; MAGNETIC-RESONANCE; FORMALIN FIXATION; RELAXATION-TIMES; POSTMORTEM; VALIDATION; NUCLEUS;
D O I
10.3389/fnana.2020.536838
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Post mortemmagnetic resonance imaging (MRI) studies on the human brain are of great interest for the validation ofin vivoMRI. It facilitates a link between functional and anatomical information available from MRIin vivoand neuroanatomical knowledge available from histology/immunocytochemistry. However, linkingin vivoandpost mortemMRI to microscopy techniques poses substantial challenges. Fixation artifacts and tissue deformation of extracted brains, as well as co registration of 2D histology to 3D MRI volumes complicate direct comparison between modalities. Moreover,post mortembrain tissue does not have the same physical properties asin vivotissue, and therefore MRI approaches need to be adjusted accordingly. Here, we present a pipeline in which whole-brain humanpost mortem in situMRI is combined with subsequent tissue processing of the whole human brain, providing a 3-dimensional reconstruction via blockface imaging. To this end, we adapted tissue processing procedures to allow bothpost mortemMRI and subsequent histological and immunocytochemical processing. For MRI, tissue was packed in a susceptibility matched solution, tailored to fit the dimensions of the MRI coil. Additionally, MRI sequence parameters were adjusted to accommodate T1 and T2*shortening, and scan time was extended, thereby benefiting the signal-to-noise-ratio that can be achieved using extensive averaging without motion artifacts. After MRI, the brain was extracted from the skull and subsequently cut while performing optimized blockface imaging, thereby allowing three-dimensional reconstructions. Tissues were processed for Nissl and silver staining, and co-registered with the blockface images. The combination of these techniques allows direct comparisons across modalities.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] Single subject and group whole-brain fMRI mapping of male genital sensation at 7 Tesla
    Sven P. R. Luijten
    Ilse M. Groenendijk
    Joan C. Holstege
    Chris I. De Zeeuw
    Wietske van der Zwaag
    Bertil F. M. Blok
    Scientific Reports, 10
  • [22] Alzheimer's Disease Detection Through Whole-Brain 3D-CNN MRI
    Folego, Guilherme
    Weiler, Marina
    Casseb, Raphael F.
    Pires, Ramon
    Rocha, Anderson
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2020, 8
  • [23] Multimodal MRI-Based Whole-Brain Assessment in Patients In Anoxoischemic Coma by Using 3D Convolutional Neural Networks
    Giulia Maria Mattia
    Benjamine Sarton
    Edouard Villain
    Helene Vinour
    Fabrice Ferre
    William Buffieres
    Marie-Veronique Le Lann
    Xavier Franceries
    Patrice Peran
    Stein Silva
    Neurocritical Care, 2022, 37 : 303 - 312
  • [24] Multimodal MRI-Based Whole-Brain Assessment in Patients In Anoxoischemic Coma by Using 3D Convolutional Neural Networks
    Mattia, Giulia Maria
    Sarton, Benjamine
    Villain, Edouard
    Vinour, Helene
    Ferre, Fabrice
    Buffieres, William
    Le Lann, Marie-Veronique
    Franceries, Xavier
    Peran, Patrice
    Silva, Stein
    NEUROCRITICAL CARE, 2022, 37 (SUPPL 2) : 303 - 312
  • [25] Whole-brain 3D mapping of oxygen metabolism using constrained quantitative BOLD
    Lee, Hyunyeol
    Wehrli, Felix W.
    NEUROIMAGE, 2022, 250
  • [26] A Systematic Pipeline for the Objective Comparison of Whole-Brain Spectroscopic MRI with Histology in Biopsy Specimens from Grade 3 Glioma
    Cordova, J. Scott
    Gurbani, Saumya S.
    Olson, Jeffrey J.
    Liang, Zhongxing
    Cooper, Lee A. D.
    Shu, Hui-Kuo G.
    Schreibmann, Eduard
    Neill, Stewart G.
    Hadjipanayis, Constantinos G.
    Holder, Chad A.
    Shim, Hyunsuk
    TOMOGRAPHY, 2016, 2 (02) : 106 - 116
  • [27] 3D Reconstructions of Brain from MRI Scans Using Neural Radiance Fields
    Iddrisu, Khadija
    Malec, Sylwia
    Crimi, Alessandro
    ARTIFICIAL INTELLIGENCE AND SOFT COMPUTING, ICAISC 2023, PT II, 2023, 14126 : 207 - 218
  • [28] Whole-brain background-suppressed pCASL MRI with 1D-accelerated 3D RARE Stack-Of-Spirals readout
    Vidorreta, Marta
    Wang, Ze
    Chang, Yulin V.
    Wolk, David A.
    Fernandez-Seara, Maria A.
    Detre, John A.
    PLOS ONE, 2017, 12 (08):
  • [29] Whole-brain 3D MR fingerprinting brain imaging: clinical validation and feasibility to patients with meningioma
    Mostardeiro, Thomaz R.
    Panda, Ananya
    Witte, Robert J.
    Campeau, Norbert G.
    McGee, Kiaran P.
    Sui, Yi
    Lu, Aiming
    MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, 2021, 34 (05) : 697 - 706
  • [30] Whole-brain 3D MR fingerprinting brain imaging: clinical validation and feasibility to patients with meningioma
    Thomaz R. Mostardeiro
    Ananya Panda
    Robert J. Witte
    Norbert G. Campeau
    Kiaran P. McGee
    Yi Sui
    Aiming Lu
    Magnetic Resonance Materials in Physics, Biology and Medicine, 2021, 34 : 697 - 706