DNA pedigree;
enrichment;
parentage;
pearl culture;
population genetics;
D O I:
10.1111/j.1471-8286.2006.01362.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Six di-, tri- and tetranucleotide microsatellite loci were developed for the silver- or gold-lipped pearl oyster Pinctada maxima using a linker-ligated, magnetic bead enrichment protocol. Based on a minimum of 134 Indonesian pearl oyster samples, number of alleles and observed heterozygosity at each locus ranged from six to 17 alleles and from 0.172 to 0.813 (mean = 0.448), respectively. Mean polymorphic information content for the six loci was 0.562. These loci should be very useful in DNA parentage analyses and population differentiation of P. maxima in Australia and Indonesia.