Increased protein carbonylation of red blood cell membrane in diabetic retinopathy

被引：24

作者：

Margetis, Panagiotis I. ^{[1
]}

Antonelou, Marianna H. ^{[1
]}

Petropoulos, Ioannis K. ^{[2
]}

Margaritis, Lukas H. ^{[1
]}

Papassideri, Issidora S. ^{[1
]}

机构：

[1] Univ Athens, Fac Biol, Dept Cell Biol & Biophys, GR-15784 Athens, Greece

[2] Univ Hosp Geneva, Dept Ophthalmol, CH-1211 Geneva 14, Switzerland

来源：

EXPERIMENTAL AND MOLECULAR PATHOLOGY | 2009年 / 87卷 / 01期

关键词：

Red blood cell membrane; Diabetes mellitus; Diabetic retinopathy; Proliferative diabetic retinopathy; Non-proliferative diabetic retinopathy; Neovascularization; Protein carbonyls; Oxidation; OXIDATIVE STRESS; ERYTHROCYTE-MEMBRANE; GLYCOSYLATION; PRODUCTS; MELLITUS; PERTURBATION; PEROXIDATION; IMPAIRMENT; PLASMA; DAMAGE;

D O I：

10.1016/j.yexmp.2009.04.001

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We investigated the protein carbonylation of red blood cell (RBC) membrane in type 2 diabetic patients and the potential implication of carbonyl/oxidative stress in reflecting disease severity. Sixty-four diabetic patients with or without retinopathy of variable clinical severity (Groups DR and DM, respectively) and 20 healthy controls were included in the study. Protein carbonyls were determined in RBC membranes by immunoblotting. Compared to healthy volunteers, the RBC membranes of diabetic patients were characterized by significantly increased levels of carbonylated proteins. The carbonylation of Group DR was higher compared to that of Group DM. The subgroup of patients with proliferative retinopathy exhibited a trend towards a significant increase in protein carbonyls, compared to both free-of-retinopathy diabetic cases and non-proliferative diabetic retinopathy cases. The correlation between the chemical modifications of the erythrocyte membrane proteins and the clinical severity of diabetic retinopathy suggests a potential utility of membrane carbonylation as a marker and risk factor in the development of retinopathy. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：76 / 82

页数：7

共 50 条

[21] MEMBRANE ATTACHMENT SITES FOR THE MEMBRANE CYTOSKELETAL PROTEIN 4.1 OF THE RED-BLOOD-CELL
PINDER, JC
CHUNG, A
REID, ME
GRATZER, WB
BLOOD, 1993, 82 (11) : 3482 - 3488
[22] LIPID AND PROTEIN CONTRIBUTION TO RED BLOOD-CELL MEMBRANE VISCOELASTICITY
CHABANEL, A
CLINICAL HEMORHEOLOGY, 1988, 8 (3-4): : 307 - 318
[23] LIPID AND PROTEIN CONTRIBUTION TO RED BLOOD-CELL MEMBRANE VISCOELASTICITY
CHABANEL, A
CLINICAL HEMORHEOLOGY, 1987, 7 (06): : 873 - 873
[24] Protein disregulation in red blood cell membranes of type 2 diabetic patients
Jiang, M
Jia, LZ
Jiang, WH
Hu, XJ
Zhou, HQ
Gao, X
Lu, ZQ
Zhang, ZH
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 309 (01) : 196 - 200
[25] ASSOCIATION BETWEEN RED BLOOD CELL DEFORMABILITY AND DIABETIC RETINOPATHY IN PATIENTS WITH TYPE 2 DIABETES MELLITUS
Won, K. C.
Moon, J. S.
Yoon, J. S.
Lee, H. W.
DIABETES RESEARCH AND CLINICAL PRACTICE, 2014, 106 : S92 - S92
[26] RED BLOOD-CELL AGGREGABILITY IN DIABETES-MELLITUS - WITH SPECIAL REFERENCE TO DIABETIC-RETINOPATHY
TANAHASHI, N
GOTOH, F
TOMITA, M
NARA, M
MIYASHITA, K
CLINICAL HEMORHEOLOGY, 1988, 8 (06): : 973 - 973
[27] INCREASED INCIDENCE OF DIABETIC-RETINOPATHY WITH ELEVATED BLOOD-PRESSURE
KNOWLER, WC
BENNETT, PH
BALLINTINE, EJ
MILLER, M
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1978, 108 (03) : 240 - 240
[28] INCREASED RIGIDITY OF RED BLOOD-CELL MEMBRANE IN YOUNG SPONTANEOUSLY HYPERTENSIVE RATS
CHABANEL, A
SCHACHTER, D
CHIEN, S
HYPERTENSION, 1987, 10 (06) : 603 - 607
[29] Malaria and the red blood cell membrane
Cooke, BM
Mohandas, N
Coppel, RL
SEMINARS IN HEMATOLOGY, 2004, 41 (02) : 173 - 188
[30] Red blood cell membrane disorders
Tse, WT
Lux, SE
BRITISH JOURNAL OF HAEMATOLOGY, 1999, 104 (01) : 2 - 13

← 1 2 3 4 5 →