An eco-friendly and sustainable process for enzymatic hydrolysis of penicillin G in cloud point system

被引：13
|
作者：
Wang, Li [1 ]
Wang, Zhilong
Xu, Jian-He
Bao, Da
Qi, Hanshi
机构：
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China
[3] Shanghai Highly Integrated Bioproc Sci & Technol, Shanghai 200238, Peoples R China
[4] Shanghai Jiao Tong Univ, Inst Biotechnol, Shanghai 201101, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2006年 / 29卷 / 03期
关键词：
nonionic surfactant; cloud point system; extractive biocatalysis; penicillin acylase; hydrolysis;
D O I：
10.1007/s00449-006-0067-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Enzymatic hydrolysis of penicillin G by immobilized penicillin acylase in a nonionic surfactant mediated cloud point system was presented. The effect of the operation parameters on equilibrium pH of this enzymatic hydrolysis process without pH control was examined. A relatively high equilibrium pH in cloud point system without pH control can be obtained. The feasibility of recycling utilization of the nonionic surfactant, a novel green solvent, was also investigated experimentally. Enzymatic hydrolysis of penicillin G in a discrete semi-batch mode, which simulates a semi-continuous process., envisages a completely eco-friendly, sustainable and efficient process for production of 6-aminopenicillanic acid.
引用
收藏
页码：157 / 162
页数：6
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