mRNA stockpile and egg quality in rainbow trout (Oncorhynchus mykiss)

被引：7
|
作者：
Aegerter, S [1 ]
Jalabert, B [1 ]
Bobe, J [1 ]
机构：
[1] INRA, SCRIBE, F-35042 Rennes, France
来源：
FISH PHYSIOLOGY AND BIOCHEMISTRY | 2003年 / 28卷 / 1-4期
关键词：
Present Experiment; Rainbow Trout; Early Development; Individual Female; Oncorhynchus Mykiss;
D O I：
10.1023/B:FISH.0000030568.30472.71
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present experiment was designed in order to investigate possible correlations between the mRNA stockpile of specific transcripts and the developmental competence of the rainbow trout oocyte, estimated through embryonic survival and malformation occurrence. Post-ovulatory ageing was used as a tool to generate spawns of varying quality. Therefore, oocytes were sampled from individual females 5 or 21 days following ovulation, fertilized and incubated at 10 degreesC. Early development was monitored while the mRNA stockpile of each candidate transcripts was measured by real time RT-PCR. Results suggest that oocyte maternally-derived stores of IGF-I and IGF-II could possibly be used as markers of egg quality after post-ovulatory ageing.
引用
收藏
页码：317 / 318
页数：2
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