Fluorescence Detection of DNA Hybridization Based on the Aggregation-Induced Emission of a Perylene-Functionalized Polymer

被引：35

作者：

Wang, Guojie ^{[1
]}

Zhang, Ruichen ^{[1
]}

Xu, Chen ^{[1
]}

Zhou, Ruyi ^{[1
]}

Dong, Jie ^{[1
]}

Bai, Huitao ^{[2
]}

Zhan, Xiaowei ^{[2
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China

[2] Peking Univ, Dept Mat Sci & Engn, Coll Engn, Beijing 100871, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 14期

基金：

中国国家自然科学基金;

关键词：

aggregation-induced emission; fluorescence; hybridization; polyelectrolyte; probe; TURN-ON DETECTION; PYRENE; MULTICOLOR; COMPLEXES; AMMONIUM; BINDING; PROBES; BEACON; ASSAY; RNA;

D O I：

10.1021/am503171s

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A perylene-functionalized polycation was synthesized by quaternization of poly(4-vinylpyridine) with bromomethyl-perylene and methyl iodide, which exhibited a unique aggregation-induced emission (AIE) effect. The synthesized polycation and polyanion ssDNA could form a complex probe to detect DNA hybridization. Upon adding noncomplementary ssDNA, the fluorescence of the complex probe increased due to the AIE effect; upon adding complementary ssDNA, the fluorescence intensity changed little due to the combined effects of AIE and duplex-quenching resulting from the intercalation of perylene into the duplex.

引用

页码：11136 / 11141

页数：6

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