Gene edited rats by delivery of Cas9 protein using electroporation

被引:0
|
作者
Remy, Severine [1 ,2 ]
Quillaud-Chenouard, Vanessa [1 ,2 ]
Tesson, Laurent [1 ,2 ]
Menoret, Severine [1 ,2 ]
Usal, Claire [1 ,2 ]
Brusselle, Lucas [1 ,2 ]
Tuan Huan Nguyen [1 ,3 ]
De Cian, Anne [4 ]
Giovannangeli, Carine [4 ]
Concordet, Jean-Paul [4 ]
Anegon, Ignacio [1 ,2 ]
机构
[1] CHU Nantes, INSERM, ITUN, UMR 1064, F-44093 Nantes, France
[2] Platform Transgen Rats & ImmunoPhenom, F-44093 Nantes, France
[3] Platform GenoCellEdit Nantes, F-44093 Nantes, France
[4] Museum Natl Hist Naturelle, CNRS, INSERM, U565,UMR7196, F-75005 Paris, France
关键词
genome editing; Cas9; protein; electroporation; HDR; rats;
D O I
暂无
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:258 / 258
页数:1
相关论文
共 50 条
  • [41] Expanding the cell quantity of CRISPR/Cas9 gene editing by continuous microfluidic electroporation chip
    Li, Zixi
    Su, Xinyue
    Lin, Yihong
    Zhang, Yu
    Zhang, Anlan
    Wu, Xin
    Jiyu, Xi
    Li, Qin
    Wei, Zewen
    BIOELECTROCHEMISTRY, 2025, 161
  • [42] CRISPR/Cas9 gene-edited RPE cells to model early stages of AMD
    Fernandez-Godino, Rosario
    Bujakowska, Kinga Maria
    Pierce, Eric A.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2017, 58 (08)
  • [43] Editor’s comment on “CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein”
    Stefan Hohmann
    Molecular Genetics and Genomics, 2017, 292 : 535 - 536
  • [44] Editor's comment on "CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein"
    Hohmann, Stefan
    MOLECULAR GENETICS AND GENOMICS, 2017, 292 (03) : 535 - 536
  • [45] Electroporation Delivery of Cas9 sgRNA Ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes
    Pi, Wenhui
    Feng, Guangyu
    Liu, Minghui
    Nie, Cunxi
    Chen, Cheng
    Wang, Jingjing
    Wang, Limin
    Wan, Pengcheng
    Liu, Changbin
    Liu, Yi
    Zhou, Ping
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (17)
  • [46] Electroporation-mediated Delivery of Cas9 Ribonucleoproteins and mRNA into Freshly Isolated Primary Mouse Hepatocytes
    Rathbone, Tanner
    Ates, Ilayda
    Stuart, Callie
    Parker, Tina
    Cottle, Renee N.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (184):
  • [47] Generating rats with conditional alleles using CRISPR/Cas9
    Yuanwu Ma
    Xu Zhang
    Bin Shen
    Yingdong Lu
    Wei Chen
    Jing Ma
    Lin Bai
    Xingxu Huang
    Lianfeng Zhang
    Cell Research, 2014, 24 : 122 - 125
  • [48] Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes
    Maki Hirata
    Fuminori Tanihara
    Manita Wittayarat
    Takayuki Hirano
    Nhien Thi Nguyen
    Quynh Anh Le
    Zhao Namula
    Masahiro Nii
    Takeshige Otoi
    In Vitro Cellular & Developmental Biology - Animal, 2019, 55 : 237 - 242
  • [49] Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes
    Hirata, Maki
    Tanihara, Fuminori
    Wittayarat, Manita
    Hirano, Takayuki
    Nhien Thi Nguyen
    Quynh Anh Le
    Namula, Zhao
    Nii, Masahiro
    Otoi, Takeshige
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2019, 55 (04) : 237 - 242
  • [50] Generating rats with conditional alleles using CRISPR/Cas9
    Ma, Yuanwu
    Zhang, Xu
    Shen, Bin
    Lu, Yingdong
    Chen, Wei
    Ma, Jing
    Bai, Lin
    Huang, Xingxu
    Zhang, Lianfeng
    CELL RESEARCH, 2014, 24 (01) : 122 - 125