Gene edited rats by delivery of Cas9 protein using electroporation

被引:0
|
作者
Remy, Severine [1 ,2 ]
Quillaud-Chenouard, Vanessa [1 ,2 ]
Tesson, Laurent [1 ,2 ]
Menoret, Severine [1 ,2 ]
Usal, Claire [1 ,2 ]
Brusselle, Lucas [1 ,2 ]
Tuan Huan Nguyen [1 ,3 ]
De Cian, Anne [4 ]
Giovannangeli, Carine [4 ]
Concordet, Jean-Paul [4 ]
Anegon, Ignacio [1 ,2 ]
机构
[1] CHU Nantes, INSERM, ITUN, UMR 1064, F-44093 Nantes, France
[2] Platform Transgen Rats & ImmunoPhenom, F-44093 Nantes, France
[3] Platform GenoCellEdit Nantes, F-44093 Nantes, France
[4] Museum Natl Hist Naturelle, CNRS, INSERM, U565,UMR7196, F-75005 Paris, France
关键词
genome editing; Cas9; protein; electroporation; HDR; rats;
D O I
暂无
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:258 / 258
页数:1
相关论文
共 50 条
  • [1] Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation
    Séverine Remy
    Vanessa Chenouard
    Laurent Tesson
    Claire Usal
    Séverine Ménoret
    Lucas Brusselle
    Jean-Marie Heslan
    Tuan Huan Nguyen
    Jeremy Bellien
    Jean Merot
    Anne De Cian
    Carine Giovannangeli
    Jean-Paul Concordet
    Ignacio Anegon
    Scientific Reports, 7
  • [2] Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation
    Remy, Severine
    Chenouard, Vanessa
    Tesson, Laurent
    Usal, Claire
    Menoret, Severine
    Brusselle, Lucas
    Heslan, Jean-Marie
    Tuan Huan Nguyen
    Bellien, Jeremy
    Merot, Jean
    De Cian, Anne
    Giovannangeli, Carine
    Concordet, Jean-Paul
    Anegon, Ignacio
    SCIENTIFIC REPORTS, 2017, 7
  • [3] Direct Cas9 protein delivery mediated gene editing in the retina
    Pulman, J.
    Botto, C.
    Malki, H.
    Ren, D.
    Oudin, P.
    De Cian, A.
    As, M.
    Saubamea, B.
    Fradot, V.
    El-Amraoui, A.
    Concordet, J. P.
    Dalkara, D.
    HUMAN GENE THERAPY, 2024, 35 (3-4) : A184 - A184
  • [4] Optimization of Electroporation Conditions for Cas9 RNP Mediated Gene Editing
    Chen, Jian
    Chen, Youbang
    Sun, George
    MOLECULAR THERAPY, 2020, 28 (04) : 282 - 283
  • [5] Electroporation of CRISPR/Cas9 for efficient gene disruption in pig zygotes
    Park, I.
    Ross, P. J.
    TRANSGENIC RESEARCH, 2020, 29 (04) : 482 - 483
  • [6] Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation
    Hirata, Maki
    Wittayarat, Manita
    Namula, Zhao
    Quynh Anh Le
    Lin, Qingyi
    Nhien Thi Nguyen
    Takebayashi, Koki
    Sato, Yoko
    Tanihara, Fuminori
    Otoi, Takeshige
    MOLECULAR BIOLOGY REPORTS, 2020, 47 (07) : 5073 - 5079
  • [7] Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation
    Maki Hirata
    Manita Wittayarat
    Zhao Namula
    Quynh Anh Le
    Qingyi Lin
    Nhien Thi Nguyen
    Koki Takebayashi
    Yoko Sato
    Fuminori Tanihara
    Takeshige Otoi
    Molecular Biology Reports, 2020, 47 : 5073 - 5079
  • [8] Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes
    Rathbone, Tanner
    Ates, Ilayda
    Fernando, Lawrence
    Addlestone, Ethan
    Lee, Ciaran M.
    Richards, Vincent P.
    Cottle, Renee N.
    CRISPR JOURNAL, 2022, 5 (03): : 397 - 409
  • [9] CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein
    Lichun Tang
    Yanting Zeng
    Hongzi Du
    Mengmeng Gong
    Jin Peng
    Buxi Zhang
    Ming Lei
    Fang Zhao
    Weihua Wang
    Xiaowei Li
    Jianqiao Liu
    Molecular Genetics and Genomics, 2017, 292 : 525 - 533
  • [10] CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein
    Tang, Lichun
    Zeng, Yanting
    Du, Hongzi
    Gong, Mengmeng
    Peng, Jin
    Zhang, Buxi
    Lei, Ming
    Zhao, Fang
    Wang, Weihua
    Li, Xiaowei
    Liu, Jianqiao
    MOLECULAR GENETICS AND GENOMICS, 2017, 292 (03) : 525 - 533