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Human Platelet Lysate as a Functional Substitute for Fetal Bovine Serum in the Culture of Human Adipose Derived Stromal/Stem Cells
被引:36
|作者:
Cowper, Mathew
[1
,7
]
Frazier, Trivia
[1
,2
,3
]
Wu, Xiying
[2
,3
]
Curley, J. Lowry
[2
,4
]
Ma, Michelle H.
[3
]
Mohiuddin, Omair A.
[1
]
Dietrich, Marilyn
[5
]
McCarthy, Michelle
[1
]
Bukowska, Joanna
[1
,6
]
Gimble, Jeffrey M.
[1
,2
,3
]
机构:
[1] Tulane Univ, Sch Med, New Orleans, LA 70112 USA
[2] LaCell LLC, New Orleans, LA 70148 USA
[3] Obatala Sci Inc, New Orleans, LA 70148 USA
[4] Axosim Inc, New Orleans, LA 70112 USA
[5] Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA
[6] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10748 Olsztyn, Poland
[7] Wake Forest Univ, Bowman Gray Sch Med, Dept Urol, Winston Salem, NC 27101 USA
来源:
关键词:
adipogenesis;
adipose-derived stromal/stem cells;
chondrogenesis;
colony forming unit-fibroblast;
fetal bovine serum;
human platelet lysate;
mesenchymal stem cell;
osteogenesis;
regenerative medicine;
MESENCHYMAL STEM-CELLS;
VASCULAR FRACTION;
COLONY FORMATION;
EXPANSION;
DIFFERENTIATION;
PROLIFERATION;
SECRETOME;
D O I:
10.3390/cells8070724
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Introduction: Adipose derived stromal/stem cells (ASCs) hold potential as cell therapeutics for a wide range of disease states; however, many expansion protocols rely on the use of fetal bovine serum (FBS) as a cell culture nutrient supplement. The current study explores the substitution of lysates from expired human platelets (HPLs) as an FBS substitute. Methods: Expired human platelets from an authorized blood center were lysed by freeze/thawing and used to examine human ASCs with respect to proliferation using hematocytometer cell counts, colony forming unit-fibroblast (CFU-F) frequency, surface immunophenotype by flow cytometry, and tri-lineage (adipocyte, chondrocyte, osteoblast) differentiation potential by histochemical staining. Results: The proliferation assays demonstrated that HPLs supported ASC proliferation in a concentration dependent manner, reaching levels that exceeded that observed in the presence of 10% FBS. The concentration of 0.75% HPLs was equivalent to 10% FBS when utilized in cell culture media with respect to proliferation, immunophenotype, and CFU-F frequency. When added to osteogenic, adipogenic, and chondrogenic differentiation media, both supplements showed appropriate differentiation by staining. Conclusion: HPLs is an effective substitute for FBS in the culture, expansion and differentiation of human ASCs suitable for pre-clinical studies; however, additional assays and analyses will be necessary to validate HPLs for clinical applications and regulatory approval.
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页数:13
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