An integration-free, virus-free rhesusmacaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

被引：6
|
作者：
Sosa, Enrique [1 ]
Kim, Rachel [1 ]
Rojas, Ernesto J. [1 ]
Hosohama, Linzi [1 ]
Hennebold, Jon D. [3 ,4 ]
Orwig, Kyle E. [2 ]
Clark, Amander T. [1 ]
机构：
[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA 90095 USA
[2] Univ Pittsburgh, Sch Med, Magee Womans Res Inst, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA 15213 USA
[3] Oregon Natl Primate Res Ctr, Div Reprod & Dev Sci, Beaverton, OR USA
[4] Oregon Hlth & Sci Univ, Oregon Dept Obstet & Gynecol, Beaverton, OR 97006 USA
来源：
STEM CELL RESEARCH | 2017年 / 21卷
关键词：
D O I：
10.1016/j.scr.2017.03.011
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016). (C) 2017 The Authors. Published by Elsevier B.V.
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收藏
页码：5 / 8
页数：4
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