Oligomerization of Cry11Aa from Bacillus thuringiensis Has an Important Role in Toxicity against Aedes aegypti

被引：22
|
作者：
Munoz-Garay, Carlos [1 ]
Rodriguez-Almazan, Claudia [1 ]
Aguilar, Jose N. [1 ]
Portugal, Leivi [1 ]
Gomez, Isabel [1 ]
Saab-Rincon, Gloria [1 ]
Soberon, Mario [1 ]
Bravo, Alejandra [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62250, Morelos, Mexico
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 23期
关键词：
CIRCULAR-DICHROISM SPECTRA; PRE-PORE; CULEX-QUINQUEFASCIATUS; SECONDARY STRUCTURE; SUBSP ISRAELENSIS; CRYSTAL PROTEINS; CRY1AB TOXIN; CYTA; RECEPTOR; CRYIVD;
D O I：
10.1128/AEM.01303-09
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cry11Aa and Cyt1Aa of Bacillus thuringiensis are active against mosquitoes and show synergism. Cyt1Aa functions as a membrane receptor inducing Cry11Aa oligomerization. Here we characterized Cry11Aa helix alpha-3 mutants impaired in oligomerization and toxicity against Aedes aegypti, indicating that oligomerization of Cry11Aa is important for toxin action. Cyt1Aa did not recover the insecticidal activity of Cry11Aa mutants.
引用
收藏
页码：7548 / 7550
页数：3
相关论文
共 50 条
  • [31] A GPI-anchored alkaline phosphatase is a functional midgut receptor of Cry11Aa toxin in Aedes aegypti larvae
    Fernandez, LE
    Aimanova, KG
    Gill, SS
    Bravo, A
    Soberón, M
    BIOCHEMICAL JOURNAL, 2006, 394 (01) : 77 - 84
  • [32] Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp israelensis in Asticcacaulis excentricus
    Zheng, Dasheng
    Valdez-Cruz, Norma Adriana
    Armengol, Gemma
    Sevrez, Chloe
    Munoz-Olaya, Jose Maurilio
    Yuan, Zhiming
    Orduz, Sergio
    Crickmore, Neil
    CURRENT MICROBIOLOGY, 2007, 54 (01) : 58 - 62
  • [33] Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)
    da Costa Fernandes, Geysla
    de Prado Costa, Dalton Kaynnan
    de Oliveira, Nayanne Santos
    de Sousa, Emanuelle Cristine Pereira
    Machado, Deborah Heloisa Bittencourt
    Polanczyk, Ricardo Antonio
    de Siqueira, Herbert Alvaro Abreu
    da Silva, Maria Cleoneide
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [34] Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin
    Chen, Jianwu
    Aimanova, Karlygash G.
    Pan, Songqin
    Gill, Sarjeet S.
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2009, 39 (10) : 688 - 696
  • [35] Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)
    Geysla da Costa Fernandes
    Dalton Kaynnan de Prado Costa
    Nayanne Santos de Oliveira
    Emanuelle Cristine Pereira de Sousa
    Déborah Heloísa Bittencourt Machado
    Ricardo Antonio Polanczyk
    Herbert Álvaro Abreu de Siqueira
    Maria Cleoneide da Silva
    Scientific Reports, 12
  • [36] Co-Expression of the Mosquitocidal Toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis Subsp. israelensis in Asticcacaulis excentricus
    Dasheng Zheng
    Norma Adriana Valdez-Cruz
    Gemma Armengol
    Chloe Sevrez
    Jose Maurilio Munoz-Olaya
    Zhiming Yuan
    Sergio Orduz
    Neil Crickmore
    Current Microbiology, 2007, 54 : 58 - 62
  • [37] Bacillus thuringiensis subsp israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor
    Pérez, C
    Fernandez, LE
    Sun, JG
    Folch, JL
    Gill, SS
    Soberón, M
    Bravo, A
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (51) : 18303 - 18308
  • [38] Aedes cadherin receptor that mediates Bacillus thuringiensis Cry11A toxicity is essential for mosquito development
    Chen, Jianwu
    Aimanova, Karly G.
    Gill, Sarjeet S.
    PLOS NEGLECTED TROPICAL DISEASES, 2020, 14 (02):
  • [39] Identification of Aedes aegypti specificity motifs in the N-terminus of the Bacillus thuringiensis Cry2Aa pesticidal protein
    Goje, Lazarus Joseph
    Elmi, Elmi Dahir
    Bracuti, Anthony
    Courty, Thomas
    Rao, Tejas
    Alzahrani, Faisal Ay
    Crickmore, Neil
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2020, 174
  • [40] Cloning and Epitope Mapping of Cry11Aa-Binding Sites in the Cry11Aa-Receptor Alkaline Phosphatase from Aedes aegypti
    Fernandez, Luisa E.
    Martinez-Anaya, Claudia
    Lira, Erandi
    Chen, Jianwu
    Evans, Amy
    Hernandez-Martinez, Salvador
    Bravo, Alejandra
    Gill, Sarjeet S.
    Soberon, Mario
    BIOCHEMISTRY, 2009, 48 (37) : 8899 - 8907
12345