A microfluidic-based system for analysis of single cells based on Ca2+ flux

被引：27
|
作者：
Zhang, Xunli
Yin, Huabing
Cooper, Jon M.
Haswell, Stephen J. [1 ]
机构：
[1] Univ Hull, Dept Chem, Kingston Upon Hull HU6 7RX, N Humberside, England
[2] Univ Glasgow, Dept Elect & Elect Engn, Glasgow G12 8QQ, Lanark, Scotland
来源：
ELECTROPHORESIS | 2006年 / 27卷 / 24期
关键词：
calcium flux; CHOK-1; cells; fluorescent measurement; microfluidics; lab-on-a-chip;
D O I：
10.1002/elps.200600390
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.
引用
收藏
页码：5093 / 5100
页数：8
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