Paeonol triggers apoptosis in HeLa cervical cancer cells: the role of mitochondria-related caspase pathway

被引:19
|
作者
Du, Jikun [2 ]
Song, Daibo [1 ]
Li, Jinwen [1 ]
Li, Yuanhua [1 ]
Li, Baohong [1 ]
Li, Li [1 ]
机构
[1] Guangdong Med Univ, Dongguan Sci Res Ctr, Dept Pharmacol, Dongguan, Peoples R China
[2] Shajing Peoples Hosp Baoan Shenzhen, Shenzhen Hosp Integrated Tradit Chinese & Western, Peoples Hosp Baoan Shenzhen Grp 2, Cent Res Lab, Shenzhen, Peoples R China
关键词
Paeonol; HeLa cells; Apoptosis; Mitochondrial pathway; CYTOCHROME-C; INDUCTION; INHIBITION; ACTIVATION; RELEASE; GROWTH; MICE;
D O I
10.1007/s00213-021-05811-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Paeonol is a biologically active component purified from the root bark of Cortex Moutan that exerts pharmacological effects on the cervical cancer. In this study, we aim to evaluate the anti-cervical cancer capacity of paeonol and to investigate the mechanism driving its anti-cervical cancer effect. Paeonol administration markedly restrained the proliferation and caused apoptosis in HeLa cells. Furthermore, paeonol treatment resulted in a mitochondrial dysfunction in HeLa cells, including the inducing of mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, and the release of cytochrome c. Moreover, the Bcl-2/Bax proportion was obviously downregulated and cleaved caspase-3 expression was evaluated through paeonol treatment. Additionally, the expression of p-PI3K and p-Akt was noticeably reduced in response to paeonol treatment in HeLa cells. Our findings indicated that paeonol exerts an anticancer potential in HeLa cells, at least in a manner, via triggering the mitochondrial pathway of cellular apoptosis by inhibiting PI3K/Akt signaling. Thus, paeonol has great potential as a promising therapeutic compound to resist human cervical cancer.
引用
收藏
页码:2083 / 2092
页数:10
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