Expression of PD-L1 and presence of CD8-positive T cells in pre-treatment specimens of locally advanced cervical cancer

被引:138
|
作者
Enwere, Emeka K. [1 ]
Kornaga, Elizabeth N. [1 ]
Dean, Michelle [1 ]
Koulis, Theodora A. [2 ]
Tien Phan [2 ]
Kalantarian, Maria [1 ]
Kobel, Martin [3 ]
Ghatage, Prafull [2 ]
Magliocco, Anthony M. [4 ]
Lees-Miller, Susan P. [5 ]
Doll, Corinne M. [2 ]
机构
[1] Tom Baker Canc Clin, Translat Labs, Calgary, AB, Canada
[2] Tom Baker Canc Clin, Dept Oncol, 1331 29th St NW, Calgary, AB T2N 4N2, Canada
[3] Calgary Lab Serv, Dept Pathol & Lab Med, Calgary, AB, Canada
[4] H Lee Moffitt Canc Ctr & Res Inst, Dept Anat Pathol, Tampa, FL USA
[5] Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada
关键词
TUMOR-INFILTRATING LYMPHOCYTES; NEGATIVE BREAST-CANCER; LUNG-CANCER; ANTI-PD-L1; ANTIBODY; IMMUNE CHECKPOINT; CLINICAL ACTIVITY; OPEN-LABEL; CARCINOMA; MULTICENTER; HEAD;
D O I
10.1038/modpathol.2016.221
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Several of the cancer immunotherapies under investigation or in clinical use target the programmed death-ligand 1/programmed death-1 (PD-L1/PD-1) signaling axis. PD-L1 expression in tumor samples has been used as a predictive marker for response to these therapeutics, and may also have independent prognostic utility when assessed along with immune cell markers. Our objectives were to assess the expression of PD-L1 in tumor specimens from a uniformly treated patient cohort with locally advanced cervical cancer, and to determine its prognostic significance along with the density of tumor-infiltrating T cells. We identified 120 patients with locally advanced cervical cancer treated with radical chemoradiotherapy, and built tissue microarrays from their formalin-fixed, paraffin-embedded pre-treatment biopsies. We used conventional brightfield and fluorescence immunohistochemistry to detect PD-L1, and quantified protein expression using both manual pathologist scoring and automated software analysis. We also evaluated the effect of PD-L1 expression in tumors, along with the presence and density of intra-tumoral CD8(+) T cells, on patient survival outcomes. Approximately 96% of the tumor samples expressed PD-L1, as determined using quantitative software analysis. Neither expression of PD-L1 nor density of CD8(+) T cells was associated with progression-free or overall survival. However, there was a trend towards worse progression-free survival in patients whose tumors expressed PD-L1 but lacked CD8(+) T cells (hazard ratio = 0.43 (0.18-1.01), P=0.053). Nevertheless, the high percentage of cervical cancer tumor samples expressing PD-L1 suggests that anti-PD-L1 or anti-PD-1 therapies are potential treatment options for this patient population.
引用
收藏
页码:577 / 586
页数:10
相关论文
共 50 条
  • [21] PD-L1 Expression and CD8+ T Cell Infiltration Predict a Favorable Prognosis in Advanced Gastric Cancer
    Wang, Yangyang
    Zhu, Chunchao
    Song, Wei
    Li, Jun
    Zhao, Gang
    Cao, Hui
    JOURNAL OF IMMUNOLOGY RESEARCH, 2018, 2018
  • [22] T-LYMPHOCYTES INFILTRATING ADVANCED GRADES OF CERVICAL NEOPLASIA - CD8-POSITIVE CELLS ARE RECRUITED TO INVASION
    EDWARDS, RP
    KUYKENDALL, K
    CROWLEYNOWICK, P
    PARTRIDGE, EE
    SHINGLETON, HM
    MESTECKY, J
    CANCER, 1995, 76 (08) : 1411 - 1415
  • [23] Clinical Significance of PD-L1 Expression and CD8-Positive Tumor-Infiltrating Lymphocytes in Patients with Cavitary Lung Adenocarcinoma
    Liu, Jiangyong
    Gu, Mingming
    Xue, Yang
    Wang, Qiong
    Ren, Yong
    Huang, Wencai
    ONCOLOGIE, 2021, 23 (03) : 439 - 452
  • [24] Tumour CD274 (PD-L1) expression and T cells in colorectal cancer
    Masugi, Yohei
    Nishihara, Reiko
    Yang, Juhong
    Mima, Kosuke
    da Silva, Annacarolina
    Shi, Yan
    Inamura, Kentaro
    Cao, Yin
    Song, Mingyang
    Nowak, Jonathan A.
    Liao, Xiaoyun
    Nosho, Katsuhiko
    Chan, Andrew T.
    Giannakis, Marios
    Bass, Adam J.
    Hodi, F. Stephen
    Freeman, Gordon J.
    Rodig, Scott
    Fuchs, Charles S.
    Qian, Zhi Rong
    Ogino, Shuji
    GUT, 2017, 66 (08) : 1463 - 1473
  • [25] Radiotherapy Scheme Effect on PD-L1 Expression for Locally Advanced Rectal Cancer
    Boustani, Jihane
    Derangere, Valentin
    Bertaut, Aurelie
    Adotevi, Olivier
    Morgand, Veronique
    Charon-Barra, Celine
    Ghiringhelli, Francois
    Mirjolet, Celine
    CELLS, 2020, 9 (09) : 1 - 20
  • [26] PD-L1 expression and pattern of immune cells in pre-treatment specimens are associated with disease-free survival for HR-NMIBC undergoing BCG treatment
    Roumiguie, Mathieu
    Comperat, Eva
    Chaltiel, Leonor
    Nouhaud, Francois Xavier
    Verhoest, Gregory
    Masson-Lecomte, Alexandra
    Colin, Pierre
    Audenet, Francois
    Houede, Nadine
    Larre, Stephane
    Xylinas, Evanguelos
    Brunelle, Serge
    Piana-Thomassin, Jeanne
    Cotte, Juliette
    Pignot, Geraldine
    Neuzillet, Yann
    Roupret, Morgan
    WORLD JOURNAL OF UROLOGY, 2021, 39 (11) : 4055 - 4065
  • [27] PD-L1 expression and pattern of immune cells in pre-treatment specimens are associated with disease-free survival for HR-NMIBC undergoing BCG treatment
    Mathieu Roumiguié
    Eva Compérat
    Léonor Chaltiel
    François Xavier Nouhaud
    Gregory Verhoest
    Alexandra Masson-Lecomte
    Pierre Colin
    François Audenet
    Nadine Houédé
    Stéphane Larré
    Evanguelos Xylinas
    Serge Brunelle
    Jeanne Piana-Thomassin
    Juliette Cotte
    Géraldine Pignot
    Yann Neuzillet
    Morgan Rouprêt
    World Journal of Urology, 2021, 39 : 4055 - 4065
  • [28] Clinicopathologic implications of immune classification by PD-L1 expression and CD8-positive tumor-infiltrating lymphocytes in stage II and III gastric cancer patients
    Koh, Jiwon
    Ock, Chan-Young
    Kim, Jin Won
    Nam, Soo Kyung
    Kwak, Yoonjin
    Yun, Sumi
    Ahn, Sang-Hoon
    Park, Do Joong
    Kim, Hyung-Ho
    Kim, Woo Ho
    Lee, Hye Seung
    ONCOTARGET, 2017, 8 (16) : 26356 - 26367
  • [29] PD-L1 expression on pre-treatment circulating tumour cells, but not serum VEGF, is predictive of response to pembrolizumab in melanoma
    Khattak, M. A.
    Gray, E. S.
    Reid, A.
    Periera, M.
    McEvoy, A.
    Aya-Bonilla, C.
    Meniawy, T.
    Didan, A.
    Millward, M.
    Ziman, M.
    ANNALS OF ONCOLOGY, 2018, 29
  • [30] Assessment of Tumoral PD-L1 Expression and Intratumoral CD8+T Cells in Urothelial Carcinoma
    Faraj, Sheila F.
    Munari, Enrico
    Guner, Gunes
    Taube, Janis
    Anders, Robert
    Hicks, Jessica
    Meeker, Alan
    Schoenberg, Mark
    Bivalacqua, Trinity
    Drake, Charles
    Netto, George J.
    UROLOGY, 2015, 85 (03) : 703.e1 - 703.e6